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Oral Immunization with YncE-Encapsulated Chitosan Nanoparticles Confers Protective Immunity against Enterotoxigenic Escherichia coli (ETEC) in a BALB/c Murine Model | ||
| Iranian Journal of Immunology | ||
| دوره 23، شماره 3، آذر 2026، صفحه 4-4 | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2026.111947.3238 | ||
| نویسندگان | ||
| Fatemeh Mahmoodi Lamooki1؛ Farajollah Shahriari Ahmadi2؛ Rouhollah Kazemi3؛ Amir Homayoun Keihan4؛ Hamidreza Raeespour5؛ Jafar Amani* 6 | ||
| 1School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 2Department of Crop Biotechnology and Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad (FUM), Mashhad, Iran. | ||
| 3Molecular Biology Research Center, Green Gene Company, Tehran, Iran. | ||
| 4Molecular Biology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran. | ||
| 5Department of Biological Sciences and Technologies, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. | ||
| 6Applied Microbiology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran. | ||
| چکیده | ||
| Background: Every year, a large number of people worldwide suffer from gastrointestinal diseases such as diarrhea. Among this population, children under five are particularly vulnerable and are at increased risk of death or malnutrition. Enterotoxigenic Escherichia coli (ETEC) is a major cause of this severe diarrhea. The bacteria first adhere to intestinal cells using colonization factors (CFs), then produce heat-stable (ST) and heat-labile (LT) toxins, which together are responsible for causing the severe diarrheal illness. Objective: to investigate the potential of the recombinant surface protein YncE, encapsulated within chitosan nanoparticles, as a novel vaccine candidate, and to evaluate its immunogenicity. Methods: The recombinant YncE gene, encoding the surface protein YncE, was cloned, expressed, purified. Successful expression and purification were confirmed via Western blot analysis. The purified recombinant protein was subsequently encapsulated within chitosan nanoparticles, and the particle size was measured. BALB/c mice were divided into five groups: recombinant protein injection, nanoparticle injection, oral nanoparticle administration, combined oral-injection nanoparticle regimen, and control. Immunogenicity was evaluated by measuring antibody titers using an enzyme-linked immunosorbent assay (ELISA). Finally, a bacterial challenge assay was conducted on all immunized groups to evaluate protective efficacy. Results: Following expression and purification, the size of the YncE-containing nanoparticles was measured. These nanoparticles successfully induced both humoral and mucosal immune responses. Furthermore, immunized mice challenged with the YncE-containing chitosan nanovaccine demonstrated protective immunity against ETEC. Conclusion: Taken together, our results show that the YncE protein-when packaged in chitosan nanoparticles-protects mice against ETEC infection. This makes YncE a strong candidate for future vaccine development. | ||
| کلیدواژهها | ||
| Chitosan nanoparticles؛ Diarrheal disease؛ Enterotoxigenic Escherichia coli؛ YncE gene | ||
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آمار تعداد مشاهده مقاله: 23 |
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