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Vakili Saatloo, Maedeh, Yari Khosroushahi, Ahmad, Mohamadzadeh Gharebaghi, Yasmin, Kouhsoltani, Maryam. (1405). ROCK1 and ROCK2 siRNAs Decrease Proliferation and Increase Apoptosis in Human Head and Neck Squamous Cell Carcinoma. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/mejc.2026.106939.2278
Maedeh Vakili Saatloo; Ahmad Yari Khosroushahi; Yasmin Mohamadzadeh Gharebaghi; Maryam Kouhsoltani. "ROCK1 and ROCK2 siRNAs Decrease Proliferation and Increase Apoptosis in Human Head and Neck Squamous Cell Carcinoma". سامانه مدیریت نشریات علمی, , , 1405, -. doi: 10.30476/mejc.2026.106939.2278
Vakili Saatloo, Maedeh, Yari Khosroushahi, Ahmad, Mohamadzadeh Gharebaghi, Yasmin, Kouhsoltani, Maryam. (1405). 'ROCK1 and ROCK2 siRNAs Decrease Proliferation and Increase Apoptosis in Human Head and Neck Squamous Cell Carcinoma', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/mejc.2026.106939.2278
Vakili Saatloo, Maedeh, Yari Khosroushahi, Ahmad, Mohamadzadeh Gharebaghi, Yasmin, Kouhsoltani, Maryam. ROCK1 and ROCK2 siRNAs Decrease Proliferation and Increase Apoptosis in Human Head and Neck Squamous Cell Carcinoma. سامانه مدیریت نشریات علمی, 1405; (): -. doi: 10.30476/mejc.2026.106939.2278

ROCK1 and ROCK2 siRNAs Decrease Proliferation and Increase Apoptosis in Human Head and Neck Squamous Cell Carcinoma

Middle East Journal of Cancer
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 09 مهر 1405    اصل مقاله (749.54 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2026.106939.2278
نویسندگان
Maedeh Vakili Saatloo1؛ Ahmad Yari Khosroushahi2؛ Yasmin Mohamadzadeh Gharebaghi3؛ Maryam Kouhsoltani* 3
1Department of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, MA, USA.
2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Background: ROCK genes play an essential role in cancer development. The aim of the present study was to investigate a novel gene therapy approach by ROCK1 and ROCK2 genes silencing for head and neck squamous cell carcinoma (HNSCC) treatment.
Method: In this in-vitro study, two cell lines including HN5 for HNSCC and a normal cell line were treated with ROCK1 and ROCK2 siRNAs together or alone with or without cisplatin. MTS, the real time polymesrae chain reaction, 4′,6-diamidino-2-phenylindole (DAPI) staining, Enzyme-linked Immunosorbent Assay (ELISA) and flow cytometry tests were carried out.
Results: The highest level of apoptosis (88%) was seen in HN5 cells treated with ROCK2 siRNA, while this treatment revealed 1.08% apoptosis in normal cells. Cell viability decreased significantly to 0.31 after ROCK2 siRNA with cisplatin treatment and to 0.33 after ROCK1 / ROCK2 siRNA with cisplatin treatment in HN5. ROCK1 / ROCK2 genes silencing (especially ROCK2) increased apoptosis and decrease proliferation in HNSCC. The combination of ROCK2 siRNA and cisplatin demonstrated the greatest effect on apoptosis of cancerous cells. However, adding cisplatin to ROCK2 siRNA did not significantly increase apoptosis.
Conclusion: Based on our in-vitro data and due to the issue of cost and benefit, ROCK2 siRNA alone represents a potent and selective agent for the induction of apoptosis and inhibition of proliferation in HNSCC.  Our findings suggest that ROCK1 / ROCK2 genes silencing (especially ROCK2) warrants further investigation as potential treatment strategy for HNSCC.  Further studies are essential to support the translational potential of ROCK-targeted siRNA therapy.

تازه های تحقیق

Maedeh Vakili Saatloo (google scholar)

Maryam Kouhsoltani (google scholar)

کلیدواژه‌ها
Apoptosis؛ Gene silencing؛ Head and neck squamous cell carcinoma؛ Proliferation؛ ROCK
مراجع
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