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Asgarian, Afrouz, Hesari, Zahra, Aghaei, Mehrdad, Faghhian, Zahra, Malakouti, Romina, Besharat, Sima, Sineh Sepehr, Koushan. (1405). Differential Expression of Visfatin, Sirtuin-1, and Interleukin-18 in Ankylosing Spondylitis Patients: A Comparative Study of Etanercept-Treated versus Newly Diagnosed Patients. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/ijms.2026.108802.4382
Afrouz Asgarian; Zahra Hesari; Mehrdad Aghaei; Zahra Faghhian; Romina Malakouti; Sima Besharat; Koushan Sineh Sepehr. "Differential Expression of Visfatin, Sirtuin-1, and Interleukin-18 in Ankylosing Spondylitis Patients: A Comparative Study of Etanercept-Treated versus Newly Diagnosed Patients". سامانه مدیریت نشریات علمی, , , 1405, -. doi: 10.30476/ijms.2026.108802.4382
Asgarian, Afrouz, Hesari, Zahra, Aghaei, Mehrdad, Faghhian, Zahra, Malakouti, Romina, Besharat, Sima, Sineh Sepehr, Koushan. (1405). 'Differential Expression of Visfatin, Sirtuin-1, and Interleukin-18 in Ankylosing Spondylitis Patients: A Comparative Study of Etanercept-Treated versus Newly Diagnosed Patients', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/ijms.2026.108802.4382
Asgarian, Afrouz, Hesari, Zahra, Aghaei, Mehrdad, Faghhian, Zahra, Malakouti, Romina, Besharat, Sima, Sineh Sepehr, Koushan. Differential Expression of Visfatin, Sirtuin-1, and Interleukin-18 in Ankylosing Spondylitis Patients: A Comparative Study of Etanercept-Treated versus Newly Diagnosed Patients. سامانه مدیریت نشریات علمی, 1405; (): -. doi: 10.30476/ijms.2026.108802.4382

Differential Expression of Visfatin, Sirtuin-1, and Interleukin-18 in Ankylosing Spondylitis Patients: A Comparative Study of Etanercept-Treated versus Newly Diagnosed Patients

Iranian Journal of Medical Sciences
مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 23 تیر 1405    اصل مقاله (729.02 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2026.108802.4382
نویسندگان
Afrouz Asgarian1؛ Zahra Hesari2؛ Mehrdad Aghaei3؛ Zahra Faghhian2؛ Romina Malakouti4؛ Sima Besharat5؛ Koushan Sineh Sepehr* 4
1Clinical Research Development Unit, Sayad Shirazi Hospital, Golestan University of Medical Sciences, Gorgan, Iran
2Metabolic Disorders Research Center, Biomedical Research Institute, Golestan University of Medical Sciences, Gorgan, Iran
3Golestan Rheumatology Research Center, Biomedical Research Institute, Golestan University of Medical Science, Gorgan, Iran
4Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
5Golestan Research Center of Gastroenterology and Hepatology, Jorjani Clinical Sciences Research Institute, Golestan University of Medical Sciences, Gorgan, Iran
چکیده
Background: Ankylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the axial joints. In response to inflammation, sirtuin-1 (SIRT-1) plays a regulatory role by modulating inflammatory signaling pathways and protecting against further tissue damage. Interleukin-18 (IL-18) is a potent pro-inflammatory cytokine released predominantly by pyroptotic immune cells. Visfatin, an adipokine secreted by lymphocytes and other immune cells, is increasingly recognized as a pro-inflammatory mediator implicated in various rheumatologic disorders. This study aimed to evaluate the expression levels of SIRT-1, IL-18, and visfatin genes in immune cells of AS patients.
Methods: In 2022, this cross-sectional study was conducted in Gorgan, Iran. Peripheral blood mononuclear cells (PBMCs) were isolated from a total of 49 AS patients, including patients undergoing treatment with three doses of etanercept and the newly diagnosed, treatment-naïve patients. Total RNA was extracted from PBMCs, and complementary DNA (cDNA) was synthesized. Gene expression levels of SIRT-1, IL-18, and visfatin were quantified using real-time PCR, and data were analyzed using SPSS v16 and GraphPad Prism 9 with Student’s t test. A significant difference was considered as P<0.05. 
Results: SIRT-1 expression was significantly lower in newly diagnosed AS patients than in the etanercept-treated group (P=0.041). Visfatin expression was significantly higher in the new case group than in the treated group (P=0.021). Similarly, IL-18 expression was significantly higher in new cases than in etanercept-treated patients (P=0.028).
Conclusion: Etanercept significantly upregulates SIRT-1 expression while suppressing IL-18 and visfatin levels in AS patients, highlighting its pleiotropic anti-inflammatory mechanisms beyond TNF-α blockade. These findings provide novel insights into the molecular pathways modulated by anti-TNF therapy, particularly the interplay between SIRT1-mediated epigenetic regulation and adipokine-driven inflammation. 

تازه های تحقیق

Afrouz Asgarian (Google Scholar)
Koushan Sineh Sepehr (Google Scholar)

کلیدواژه‌ها
Ankylosing spondylitis؛ Etanercept؛ IL-18؛ SIRT-1؛ Visfatin
مراجع
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