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Homayoon, Mahin, Mehrabani, Davood, Edalatmanesh, Mohammad Amin, Shariati, Mehrdad, Amini, Masood. (1405). In Vitro Effect of Lithium Chloride on Adipose Tissue Derived Stem Cells Proliferation and Growth Kinetic. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/ijms.2026.108735.4372
Mahin Homayoon; Davood Mehrabani; Mohammad Amin Edalatmanesh; Mehrdad Shariati; Masood Amini. "In Vitro Effect of Lithium Chloride on Adipose Tissue Derived Stem Cells Proliferation and Growth Kinetic". سامانه مدیریت نشریات علمی, , , 1405, -. doi: 10.30476/ijms.2026.108735.4372
Homayoon, Mahin, Mehrabani, Davood, Edalatmanesh, Mohammad Amin, Shariati, Mehrdad, Amini, Masood. (1405). 'In Vitro Effect of Lithium Chloride on Adipose Tissue Derived Stem Cells Proliferation and Growth Kinetic', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/ijms.2026.108735.4372
Homayoon, Mahin, Mehrabani, Davood, Edalatmanesh, Mohammad Amin, Shariati, Mehrdad, Amini, Masood. In Vitro Effect of Lithium Chloride on Adipose Tissue Derived Stem Cells Proliferation and Growth Kinetic. سامانه مدیریت نشریات علمی, 1405; (): -. doi: 10.30476/ijms.2026.108735.4372

In Vitro Effect of Lithium Chloride on Adipose Tissue Derived Stem Cells Proliferation and Growth Kinetic

Iranian Journal of Medical Sciences
مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 05 خرداد 1405    اصل مقاله (1.07 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2026.108735.4372
نویسندگان
Mahin Homayoon1؛ Davood Mehrabani* 2؛ Mohammad Amin Edalatmanesh1؛ Mehrdad Shariati3؛ Masood Amini4
1Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Biology, Zand Institute of Higher Education, Shiraz, Iran
4Laparoscopy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Adipose tissue-derived stem cells (AdSCs) are widely used for regenerative medicine purposes. There is always a need to accelerate cell proliferation and protect the cells from apoptosis when cell transplantation, especially AdSCs, is targeted. Therefore, this study evaluated the effect of lithium chloride on the proliferation and growth kinetic of AdSCs. 
Methods: The study was undertaken in the Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, in 2020. Adipose tissue specimens were provided from the abdominal region of a 35-year-old woman. In an in vitro study, human AdSCs were characterized morphologically, by osteo- and adipogenic differentiation properties, and by flow cytometry. They were later treated with lithium chloride to evaluate its effect on cell proliferation and the growth kinetics of AdSCs. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay investigated lithium chloride effect on the proliferation rate and apoptosis of AdSCs. Quantitative Real Time Polymerase Chain Reaction (qPCR) was carried out to determine the expression of apoptosis genes of Apoptosis Regulator BCL2 Associated X (BAX) and B-cell lymphoma 2 (Bcl-2) protein family. 
Results: AdSCs showed mesenchymal characteristics, and lithium chloride at a dose of 6 µM had an increasing impact on cell proliferation and protected the cells from apoptosis. A non-significant increase in expression of the Bcl-2 gene (P=0.057) and a significant decrease in BAX gene (P=0.034) expression were visible. 
Conclusion: Our findings indicate that the addition of lithium chloride to the culture medium enhanced the proliferation of AdSCs and protected them against apoptosis.

تازه های تحقیق

Mahin Homayoon (Google Scholar)
Davood Mehrabani (Google Scholar)

کلیدواژه‌ها
Lithium chloride؛ Adipose tissue؛ Mesenchymal stem cells؛ Cell proliferation؛ Regenerative medicine
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