LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,264
تعداد مقالات11,528
تعداد مشاهده مقاله81,929,005
تعداد دریافت فایل اصل مقاله121,055,338
Banafi, Sirous, Marhamatizadeh, Mohammad Hossein, Tanideh, Nader, Rahimi, Ebrahim, Zare, Afshin, Safarzoda Sharoffidin, Ramazon, Tamadon, Amin. (1405). Antibacterial Potential of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Against Clostridium Perfringens: An Experimental In Vitro and In Silico Study. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/ijms.2026.108710.4369
Sirous Banafi; Mohammad Hossein Marhamatizadeh; Nader Tanideh; Ebrahim Rahimi; Afshin Zare; Ramazon Safarzoda Sharoffidin; Amin Tamadon. "Antibacterial Potential of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Against Clostridium Perfringens: An Experimental In Vitro and In Silico Study". سامانه مدیریت نشریات علمی, , , 1405, -. doi: 10.30476/ijms.2026.108710.4369
Banafi, Sirous, Marhamatizadeh, Mohammad Hossein, Tanideh, Nader, Rahimi, Ebrahim, Zare, Afshin, Safarzoda Sharoffidin, Ramazon, Tamadon, Amin. (1405). 'Antibacterial Potential of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Against Clostridium Perfringens: An Experimental In Vitro and In Silico Study', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/ijms.2026.108710.4369
Banafi, Sirous, Marhamatizadeh, Mohammad Hossein, Tanideh, Nader, Rahimi, Ebrahim, Zare, Afshin, Safarzoda Sharoffidin, Ramazon, Tamadon, Amin. Antibacterial Potential of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Against Clostridium Perfringens: An Experimental In Vitro and In Silico Study. سامانه مدیریت نشریات علمی, 1405; (): -. doi: 10.30476/ijms.2026.108710.4369

Antibacterial Potential of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Against Clostridium Perfringens: An Experimental In Vitro and In Silico Study

Iranian Journal of Medical Sciences
مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 12 اردیبهشت 1405    اصل مقاله (7.62 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2026.108710.4369
نویسندگان
Sirous Banafi1؛ Mohammad Hossein Marhamatizadeh* 1؛ Nader Tanideh2، 3؛ Ebrahim Rahimi1؛ Afshin Zare4؛ Ramazon Safarzoda Sharoffidin5؛ Amin Tamadon6، 2، 7
1Department of Food Hygiene, School of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran
2Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4International PhD Program in Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
5Department of Pharmaceutical Technology, Avicenna Tajik State Medical University, Dushanbe, Tajikistan
6Department of Natural Sciences, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
7PerciaVista R&D Co., Shiraz, Iran
چکیده
Background: Clostridium perfringens (C. perfringens) is an opportunistic anaerobic pathogen associated with severe soft tissue and gastrointestinal infections and increasing antimicrobial resistance. This study aimed to evaluate the in vitro antibacterial activity of human umbilical cord mesenchymal stem cell (hUC-MSC)-derived exosomes against C. perfringens and to explore potential exosome–enzyme interactions using in silico protein–protein docking.
Methods: This study was conducted at Shiraz University of Medical Sciences, Shiraz, Iran, in 2025. In this experimental in vitro and in silico study, human umbilical cord mesenchymal stem cells (hUC-MSCs) were isolated from term umbilical cords (UCs), characterized, and induced into osteogenic and adipogenic differentiation. Exosomes were isolated using a commercial precipitation kit and characterized by scanning electron microscopy (SEM), dynamic light scattering (DLS), and flow cytometry for CD63 expression. The antibacterial activity of exosomes against C. perfringens ATCC 2592 was evaluated by a broth microdilution minimum inhibitory concentration (MIC) assay under anaerobic conditions. Optical density (OD600) values were recorded and compared across exosome concentrations. Protein–protein docking was performed between exosomal proteins and four essential C. perfringens enzymes using ClusPro. OD600 values were compared across exosome concentrations using one-way analysis of variance (ANOVA) (P<0.05).
Results: hUC-MSCs displayed typical spindle-shaped morphology, tri-lineage differentiation potential, and a mesenchymal immunophenotype. Isolated exosomes were spherical nanovesicles (~30–100 nm) with high CD63 positivity. UC-MSC-derived exosomes inhibited C. perfringens growth in a dose-dependent manner. At 500 µg/mL, exosomes achieved 95.1% inhibition with an OD600 of 0.12±0.03 vs. 2.45±0.10 in the growth control (n=3, P<0.001). Docking analysis suggested favorable in silico interactions between Hsp70 and C. perfringens RNA polymerase and ATP synthase, and between cathelicidin and DNA gyrase, with lower but consistent docking scores for Annexin A1 across targets.
Conclusion: hUC-MSC-derived exosomes exerted significant in vitro antibacterial effects against C. perfringens and exhibited favorable in silico interactions with key bacterial enzymes.

تازه های تحقیق

Sirous Banafi (Google Scholar)
Mohammad Hossein Marhamatizadeh (Google Scholar)

کلیدواژه‌ها
Exosomes؛ Mesenchymal stem cells؛ Umbilical cord؛ Clostridium perfringens؛ Anti-bacterial agents
مراجع
  1. Ba X, Jin Y, Ning X, Gao Y, Li W, Li Y, et al. Clostridium perfringens in the Intestine: Innocent Bystander or Serious Threat? Microorganisms. 2024;12. doi: 10.3390/microorganisms12081610. PubMed PMID: 39203452; PubMed Central PMCID: PMC11356505.
  2. Yadav S, Parihar A, Sadique MA, Ranjan P, Kumar N, Singhal A, et al. Emerging point-of-care optical biosensing technologies for diagnostics of microbial infections. ACS Appl Opt Mater. 2023;1:1245-62. doi: 10.1021/acsaom.3c00129.
  3. Grenda T, Jarosz A, Sapała M, Grenda A, Patyra E, Kwiatek K. Clostridium perfringens-Opportunistic Foodborne Pathogen, Its Diversity and Epidemiological Significance. Pathogens. 2023;12. doi: 10.3390/pathogens12060768. PubMed PMID: 37375458; PubMed Central PMCID: PMC10304509.
  4. Hoseinzadeh A, Esmaeili SA, Sahebi R, Melak AM, Mahmoudi M, Hasannia M, et al. Fate and long-lasting therapeutic effects of mesenchymal stromal/stem-like cells: mechanistic insights. Stem Cell Res Ther. 2025;16:33. doi: 10.1186/s13287-025-04158-z. PubMed PMID: 39901306; PubMed Central PMCID: PMC11792531.
  5. Jiang B, Lai Y, Xiao W, Zhong T, Liu F, Gong J, et al. Microbial extracellular vesicles contribute to antimicrobial resistance. PLoS Pathog. 2024;20:e1012143. doi: 10.1371/journal.ppat.1012143. PubMed PMID: 38696356; PubMed Central PMCID: PMC11065233.
  6. Pérez J, Contreras-Moreno FJ, Marcos-Torres FJ, Moraleda-Muñoz A, Muñoz-Dorado J. The antibiotic crisis: How bacterial predators can help. Comput Struct Biotechnol J. 2020;18:2547-55. doi: 10.1016/j.csbj.2020.09.010. PubMed PMID: 33033577; PubMed Central PMCID: PMC7522538.
  7. Leiva-Sabadini C, Alvarez S, Barrera NP, Schuh C, Aguayo S. Antibacterial Effect of Honey-Derived Exosomes Containing Antimicrobial Peptides Against Oral Streptococci. Int J Nanomedicine. 2021;16:4891-900. doi: 10.2147/ijn.s315040. PubMed PMID: 34321877; PubMed Central PMCID: PMC8312616.
  8. Shaban AM, Raslan M, Sharawi ZW, Abdelhameed MS, Hammouda O, El-Masry HM, et al. Antibacterial, Antifungal, and Anticancer Effects of Camel Milk Exosomes: An In Vitro Study. Vet Sci. 2023;10. doi: 10.3390/vetsci10020124. PubMed PMID: 36851428; PubMed Central PMCID: PMC9963947.
  9. Zhankina R, Baghban N, Askarov M, Saipiyeva D, Ibragimov A, Kadirova B, et al. Mesenchymal stromal/stem cells and their exosomes for restoration of spermatogenesis in non-obstructive azoospermia: a systemic review. Stem Cell Res Ther. 2021;12:229. doi: 10.1186/s13287-021-02295-9. PubMed PMID: 33823925; PubMed Central PMCID: PMC8025392.
  10. Fathi-Karkan S, Heidarzadeh M, Narmi MT, Mardi N, Amini H, Saghati S, et al. Exosome-loaded microneedle patches: Promising factor delivery route. Int J Biol Macromol. 2023;243:125232. doi: 10.1016/j.ijbiomac.2023.125232. PubMed PMID: 37302628.
  11. Lener T, Gimona M, Aigner L, Börger V, Buzas E, Camussi G, et al. Applying extracellular vesicles based therapeutics in clinical trials - an ISEV position paper. J Extracell Vesicles. 2015;4:30087. doi: 10.3402/jev.v4.30087. PubMed PMID: 26725829; PubMed Central PMCID: PMC4698466.
  12. Tomokiyo A, Wada N, Maeda H. Periodontal Ligament Stem Cells: Regenerative Potency in Periodontium. Stem Cells Dev. 2019;28:974-85. doi: 10.1089/scd.2019.0031. PubMed PMID: 31215350.
  13. Izadi M, Dehghan Marvast L, Rezvani ME, Zohrabi M, Aliabadi A, Mousavi SA, et al. Mesenchymal Stem-Cell Derived Exosome Therapy as a Potential Future Approach for Treatment of Male Infertility Caused by Chlamydia Infection. Front Microbiol. 2021;12:785622. doi: 10.3389/fmicb.2021.785622. PubMed PMID: 35095800; PubMed Central PMCID: PMC8792933.
  14. Elnar AG, Kim GB. Complete genome sequence of Clostridium perfringens B20, a bacteriocin-producing pathogen. J Anim Sci Technol. 2021;63:1468-72. doi: 10.5187/jast.2021.e113. PubMed PMID: 34957460; PubMed Central PMCID: PMC8672250.
  15. AlJindan R, AlEraky DM, Farhat M, Almandil NB, AbdulAzeez S, Borgio JF. Genomic Insights into Virulence Factors and Multi-Drug Resistance in Clostridium perfringens IRMC2505A. Toxins (Basel). 2023;15. doi: 10.3390/toxins15060359. PubMed PMID: 37368661; PubMed Central PMCID: PMC10302382.
  16. Welsh JA, Goberdhan DCI, O’Driscoll L, Buzas EI, Blenkiron C, Bussolati B, et al. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J Extracell Vesicles. 2024;13:e12404. doi: 10.1002/jev2.12404. PubMed PMID: 38326288; PubMed Central PMCID: PMC10850029.
  17. Clinical and Laboratory Standards Institute. CLSI document M07: Performance standards for antimicrobial susceptibility testing. 10th ed. Wayne (PA): CLSI; 2009.
  18. Galvão I, de Carvalho RVH, Vago JP, Silva ALN, Carvalho TG, Antunes MM, et al. The role of annexin A1 in the modulation of the NLRP3 inflammasome. Immunology. 2020;160:78-89. doi: 10.1111/imm.13184. PubMed PMID: 32107769; PubMed Central PMCID: PMC7160667.
  19. Krasnodembskaya A, Song Y, Fang X, Gupta N, Serikov V, Lee JW, et al. Antibacterial effect of human mesenchymal stem cells is mediated in part from secretion of the antimicrobial peptide LL-37. Stem Cells. 2010;28:2229-38. doi: 10.1002/stem.544. PubMed PMID: 20945332; PubMed Central PMCID: PMC3293245.
  20. Lv H, Yuan X, Zhang J, Lu T, Yao J, Zheng J, et al. Heat shock preconditioning mesenchymal stem cells attenuate acute lung injury via reducing NLRP3 inflammasome activation in macrophages. Stem Cell Res Ther. 2021;12:290. doi: 10.1186/s13287-021-02328-3. PubMed PMID: 34001255; PubMed Central PMCID: PMC8127288.
  21. Alam SI, Bansod S, Kumar RB, Sengupta N, Singh L. Differential proteomic analysis of Clostridium perfringens ATCC13124; identification of dominant, surface and structure associated proteins. BMC Microbiol. 2009;9:162. doi: 10.1186/1471-2180-9-162. PubMed PMID: 19664283; PubMed Central PMCID: PMC2731776.
  22. Briolat V, Reysset G. Identification of the Clostridium perfringens genes involved in the adaptive response to oxidative stress. J Bacteriol. 2002;184:2333-43. doi: 10.1128/jb.184.9.2333-2343.2002. PubMed PMID: 11948145; PubMed Central PMCID: PMC134984.
  23. Hooper DC, Jacoby GA. Topoisomerase Inhibitors: Fluoroquinolone Mechanisms of Action and Resistance. Cold Spring Harb Perspect Med. 2016;6. doi: 10.1101/cshperspect.a025320. PubMed PMID: 27449972; PubMed Central PMCID: PMC5008060.
  24. Zhu JY, Yang Y, Han H, Betzi S, Olesen SH, Marsilio F, et al. Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA). J Biol Chem. 2012;287:12657-67. doi: 10.1074/jbc.M112.342725. PubMed PMID: 22378791; PubMed Central PMCID: PMC3339971.
  25. Shaaban F, Salem Sokhn E, Khalil C, Saleh FA. Antimicrobial activity of adipose-derived mesenchymal stromal cell secretome against methicillin-resistant Staphylococcus aureus. Stem Cell Res Ther. 2025;16:21. doi: 10.1186/s13287-025-04138-3. PubMed PMID: 39849590; PubMed Central PMCID: PMC11755800.
  26. Li H, Ding Y, Huang J, Zhao Y, Chen W, Tang Q, et al. Angiopep-2 Modified Exosomes Load Rifampicin with Potential for Treating Central Nervous System Tuberculosis. Int J Nanomedicine. 2023;18:489-503. doi: 10.2147/ijn.s395246. PubMed PMID: 36733407; PubMed Central PMCID: PMC9888470.
  27. Yang X, Shi G, Guo J, Wang C, He Y. Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant Staphylococcus aureus. Int J Nanomedicine. 2018;13:8095-104. doi: 10.2147/ijn.s179380. PubMed PMID: 30555228; PubMed Central PMCID: PMC6278838.
آمار
تعداد مشاهده مقاله: 6
تعداد دریافت فایل اصل مقاله: 6


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب