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Developing a Simple, Applicable, and Reliable Method to Produce and Standardize an Epsilometer (Etest) Strip for Antimicrobial Susceptibility and Minimum Inhibitory Concentration Evaluations | ||
| Trends in Pharmaceutical Sciences and Technologies | ||
| دوره 11، شماره 4 - شماره پیاپی 44، اسفند 2025 | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/tips.2026.110209.1346 | ||
| نویسندگان | ||
| Hashem Montaseri* 1؛ Fatemeh Khajeh2؛ Zahra Sobhani2؛ Amin Reza Akbarizade2؛ Marzieh Faraz2 | ||
| 1department of Quality Control, Shiraz Faculty of Pharmacy, Shiraz University of medical Sciences, Shiraz, Fars. IR | ||
| 2Department of Pharmaceutical Quality Control, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Microbial infections remain a major global health and economic burden due to increased morbidity, mortality, prolonged hospitalization, and rising antimicrobial resistance. Rapid and accurate minimum inhibitory concentration (MIC) determination is essential for effective antibiotic therapy and resistance prevention. Conventional methods, including broth micro- and macrodilution, disk diffusion, and agar well diffusion, are limited by long turnaround times, high costs, and poor quantitative precision. The Etest strip method overcomes these limitations by creating a precise antibiotic gradient on agar, delivering accurate MIC readings within 16–24 hours. However, developing countries like Iran rely entirely on imported Etest strips, facing challenges such as high costs, sanctions, currency fluctuations, and inconsistent supply. This study developed a simple, cost-effective domestic production method for Etest-like strips suitable for water-soluble antibiotics. Cellulose-based substrates with uniform weight and solvent absorption (K2) and a safe, locally available inert adhesive (G1) were used. Ciprofloxacin served as the model antibiotic, with Escherichia coli as the test organism. Drug loading and release were quantified via validated UV-Vis spectrophotometry (λ_max = 270 nm, R² = 0.9998, accuracy 97.21%, precision <3%). Prototype strips exhibited stable drug release after 20 minutes and consistent performance. MIC readings from prepared strips were compared and calibrated against CLSI-standard methods, yielding clinically equivalent results. Strips remained stable for at least 2 months at 25 ± 2 °C and 2–8 °C. This platform enables manual scaling, supports import substitution, accelerates susceptibility testing, and enhances antimicrobial stewardship in resource-limited settings. | ||
| کلیدواژهها | ||
| Epsilometer؛ Etest strip؛ Microdilution؛ MIC؛ Escherichia coli؛ ciprofloxacin | ||
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آمار تعداد مشاهده مقاله: 2 |
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