LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,262
تعداد مقالات11,502
تعداد مشاهده مقاله81,855,070
تعداد دریافت فایل اصل مقاله120,988,330
Mousazadeh, Marziyeh, Nikkhah, Maryam, Seyed, Negar, Rafati, Sima, Hosseinkhani, Saman. (1404). Efficient Generation of Bone Marrow-Derived Murine Dendritic Cells: A Protocol ‎Optimization Study. سامانه مدیریت نشریات علمی, 23(1), 3-3. doi: 10.22034/iji.2026.108143.3086
Marziyeh Mousazadeh; Maryam Nikkhah; Negar Seyed; Sima Rafati; Saman Hosseinkhani. "Efficient Generation of Bone Marrow-Derived Murine Dendritic Cells: A Protocol ‎Optimization Study". سامانه مدیریت نشریات علمی, 23, 1, 1404, 3-3. doi: 10.22034/iji.2026.108143.3086
Mousazadeh, Marziyeh, Nikkhah, Maryam, Seyed, Negar, Rafati, Sima, Hosseinkhani, Saman. (1404). 'Efficient Generation of Bone Marrow-Derived Murine Dendritic Cells: A Protocol ‎Optimization Study', سامانه مدیریت نشریات علمی, 23(1), pp. 3-3. doi: 10.22034/iji.2026.108143.3086
Mousazadeh, Marziyeh, Nikkhah, Maryam, Seyed, Negar, Rafati, Sima, Hosseinkhani, Saman. Efficient Generation of Bone Marrow-Derived Murine Dendritic Cells: A Protocol ‎Optimization Study. سامانه مدیریت نشریات علمی, 1404; 23(1): 3-3. doi: 10.22034/iji.2026.108143.3086

Efficient Generation of Bone Marrow-Derived Murine Dendritic Cells: A Protocol ‎Optimization Study

Iranian Journal of Immunology
دوره 23، شماره 1، خرداد 2026، صفحه 3-3    اصل مقاله (8.46 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2026.108143.3086
نویسندگان
Marziyeh Mousazadeh1؛ Maryam Nikkhah* 1؛ Negar Seyed2؛ Sima Rafati2؛ Saman Hosseinkhani3
1Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran‎.
2Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran.
3Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.‎
چکیده
Background: Dendritic cells (DCs) are the most potent antigen-presenting cells, playing a central role in T cell activation and the stimulation of B cell antibody production.  Owing to their immunological significance, DCs have been extensively explored for applications in cancer immunotherapy, dendritic cell–based vaccines, and strategies to overcome the immunosuppressive tumor microenvironment.
Objective: Given the broad applications of DCs and the need for efficient generation protocols, the present study aimed to optimize key parameters influencing the production of bone marrow-derived dendritic cells (BMDCs).
Methods: Variables such as mouse strain, cytokine concentrations, culture plate type, differentiation duration, incubation temperature, cell seeding density, and media replacement intervals were evaluated. CD11c expression was used as the primary marker to quantify BMDCs, while CD80 and CD86 were used as indicators of maturation status.
Results: The optimized protocol yielded 60-70% CD11c+ BMDCs and 70-80% CD80/CD86 expression within 5 days.
Conclusion: This robust and highly reproducible protocol offers valuable applications for in vitro dendritic cell generation studies.
کلیدواژه‌ها
Bone marrow-derived dendritic cells؛ Cell isolation protocols؛ CD11c؛ Cell differentiation؛ ‎Granulocyte-macrophage stimulating factor‎
آمار
تعداد مشاهده مقاله: 20
تعداد دریافت فایل اصل مقاله: 3


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب