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Zia Jahromi, Noosha, Mahmoudi Mandani, Amin, Sadeghi Moghadam Kaji, Nahid, Abbasi Teshnizi, Hadis, Nourmohammadian Dehkordi, Farnaz, Yousefi, Maryam, Sazegar, Hossein. (1404). Discovery of Biomarkers Related to Colorectal Cancer by Systematic Proteomics Analysis and Experimental Procedures.. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/ijms.2025.107064.4143
Noosha Zia Jahromi; Amin Mahmoudi Mandani; Nahid Sadeghi Moghadam Kaji; Hadis Abbasi Teshnizi; Farnaz Nourmohammadian Dehkordi; Maryam Yousefi; Hossein Sazegar. "Discovery of Biomarkers Related to Colorectal Cancer by Systematic Proteomics Analysis and Experimental Procedures.". سامانه مدیریت نشریات علمی, , , 1404, -. doi: 10.30476/ijms.2025.107064.4143
Zia Jahromi, Noosha, Mahmoudi Mandani, Amin, Sadeghi Moghadam Kaji, Nahid, Abbasi Teshnizi, Hadis, Nourmohammadian Dehkordi, Farnaz, Yousefi, Maryam, Sazegar, Hossein. (1404). 'Discovery of Biomarkers Related to Colorectal Cancer by Systematic Proteomics Analysis and Experimental Procedures.', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/ijms.2025.107064.4143
Zia Jahromi, Noosha, Mahmoudi Mandani, Amin, Sadeghi Moghadam Kaji, Nahid, Abbasi Teshnizi, Hadis, Nourmohammadian Dehkordi, Farnaz, Yousefi, Maryam, Sazegar, Hossein. Discovery of Biomarkers Related to Colorectal Cancer by Systematic Proteomics Analysis and Experimental Procedures.. سامانه مدیریت نشریات علمی, 1404; (): -. doi: 10.30476/ijms.2025.107064.4143

Discovery of Biomarkers Related to Colorectal Cancer by Systematic Proteomics Analysis and Experimental Procedures.

Iranian Journal of Medical Sciences
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 04 اسفند 1404    اصل مقاله (1.7 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/ijms.2025.107064.4143
نویسندگان
Noosha Zia Jahromi1؛ Amin Mahmoudi Mandani1؛ Nahid Sadeghi Moghadam Kaji1؛ Hadis Abbasi Teshnizi1؛ Farnaz Nourmohammadian Dehkordi2؛ Maryam Yousefi3، 4؛ Hossein Sazegar* 1
1Department of Biology, ShK.C., Islamic Azad University, Shahrekord, Iran
2Department of Biology, Science and Research Branch, Islamic Azad University of Babol, Babol, Iran
3Genetic and Biotechnology Institute, Isfahan, Iran
4Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده
Background: Long non-coding RNAs (lncRNAs) play critical roles in gene expression regulation and have emerged as potential biomarkers for cancer diagnosis and treatment. This study aimed to investigate the potential role of two lncRNAs, LINC00943 and SLC9A3-AS1, as candidate biomarkers for colorectal cancer (CRC) diagnosis and therapy.
Methods: Initially, we investigated differentially expressed proteins (DEPs) in CRC through a comprehensive bioinformatics analysis and constructed a multi-layer regulatory network to understand the complex interactions. Subsequently, tissue samples were collected from CRC patients at Milad Hospital in Isfahan, Iran, between June and December 2021. The expression levels of LINC00943 and SLC9A3-AS1 were evaluated using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Statistical analyses were performed using GraphPad Prism version 9. Expression differences between tumor and adjacent non-tumor tissues were evaluated using paired Student’s t test. Receiver operating characteristic (ROC) curve analysis was conducted to evaluate diagnostic performance. A P value less than 0.05 was considered statistically significant.
Results: LINC00943 expression was significantly upregulated in tumor tissues compared to adjacent controls (P<0.0001), with strong diagnostic performance (AUC=0.8078, sensitivity=83.3%, specificity=76.7%). SLC9A3-AS1 also showed increased expression (P=0.0299), but with limited diagnostic value (AUC=0.6124). Network analysis identified SERBP1, KHSRP, and HNRNPA1 as central hub proteins, while miR-15b-5p and NEAT1 emerged as key regulatory elements.
Conclusion: These findings suggest that LINC00943 could serve as a valuable biomarker for CRC diagnosis and treatment, providing insights into the regulatory mechanisms underlying CRC pathogenesis.

تازه های تحقیق

Noosha Zia Jahromi (Google Scholar)
Hossein Sazegar (Google Scholar)

کلیدواژه‌ها
Systems biology؛ Proteomics؛ Colorectal neoplasms؛ Biomarkers؛ Bioinformatics
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