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Fardid, Reza, Zaker, Fateme, Haghani, Masoud, Saberzadeh, Jamileh. (1405). Evaluating the Protective Effect of Melatonin on Apoptosis, Expression of Bax and Bcl2 Genes, and ROS Level after Radiofrequency Radiation on L929 Fibroblast Cell Line. سامانه مدیریت نشریات علمی, 16(2), 131-138. doi: 10.31661/jbpe.v0i0.2409-1828
Reza Fardid; Fateme Zaker; Masoud Haghani; Jamileh Saberzadeh. "Evaluating the Protective Effect of Melatonin on Apoptosis, Expression of Bax and Bcl2 Genes, and ROS Level after Radiofrequency Radiation on L929 Fibroblast Cell Line". سامانه مدیریت نشریات علمی, 16, 2, 1405, 131-138. doi: 10.31661/jbpe.v0i0.2409-1828
Fardid, Reza, Zaker, Fateme, Haghani, Masoud, Saberzadeh, Jamileh. (1405). 'Evaluating the Protective Effect of Melatonin on Apoptosis, Expression of Bax and Bcl2 Genes, and ROS Level after Radiofrequency Radiation on L929 Fibroblast Cell Line', سامانه مدیریت نشریات علمی, 16(2), pp. 131-138. doi: 10.31661/jbpe.v0i0.2409-1828
Fardid, Reza, Zaker, Fateme, Haghani, Masoud, Saberzadeh, Jamileh. Evaluating the Protective Effect of Melatonin on Apoptosis, Expression of Bax and Bcl2 Genes, and ROS Level after Radiofrequency Radiation on L929 Fibroblast Cell Line. سامانه مدیریت نشریات علمی, 1405; 16(2): 131-138. doi: 10.31661/jbpe.v0i0.2409-1828

Evaluating the Protective Effect of Melatonin on Apoptosis, Expression of Bax and Bcl2 Genes, and ROS Level after Radiofrequency Radiation on L929 Fibroblast Cell Line

Journal of Biomedical Physics and Engineering
دوره 16، شماره 2 - شماره پیاپی 1، تیر 2026، صفحه 131-138    اصل مقاله (931.11 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.2409-1828
نویسندگان
Reza Fardid1، 2؛ Fateme Zaker* 3، 2؛ Masoud Haghani1، 2؛ Jamileh Saberzadeh4
1Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Ira
4Department of Laboratory Sciences, Paramedical School of Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: The increasing use of mobile phones has raised concerns about potential health risks. In addition, the effect of protective materials is also important.
Objective: This study aimed to evaluate the protective effect of Melatonin on cell viability, apoptosis, Reactive Oxygen Species (ROS) level, and gene expression of Bax and Bcl2 in L929 fibroblast cells after Radiofrequency (RF) radiation.
Material and Methods: In this experimental study, cells were divided into six groups with at least 3 replications in each group. The two groups were respectively exposed to radiofrequency waves for 8 and 16 hours. They were pretreated with melatonin before radiation exposure, while the control group did not receive any radiation or melatonin during the experiment, and the melatonin group was just treated with melatonin.
Results: Cell survival was reduced after 8 hours of RF radiation. After 16 hours, the total apoptosis was increased, and pretreatment with melatonin increased cell survival and reduced total apoptosis. Radiation increased intracellular ROS levels. RF increased the Bax/Bcl2 ratio, and the treatment with melatonin decreased it after 8 hours. 
Conclusion: Melatonin prevents the increase in apoptosis and Bax/Bcl2 ratio, but there are no significant changes in survival and ROS levels.

تازه های تحقیق

Reza Fardid (Google Scholar)

Fateme Zaker (Google Scholar)

کلیدواژه‌ها
Radiofrequency؛ Fibroblasts؛ L929 Cells؛ Melatonin؛ Apoptosis
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