LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,248
تعداد مقالات11,353
تعداد مشاهده مقاله81,470,384
تعداد دریافت فایل اصل مقاله116,970,303
Ansari, Amir, Razzak, Zaid, Bari, Muhammad, Khan, Lubna, Amir, Quratulain, Ashraf, Armeen, Ashraf, Ajia, Haque, Zeba, Qaiser, Mahrukh, Hanif, Farina. (1405). Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/mejc.2026.105034.2219
Amir Ahmad Ansari; Zaid Abdul Razzak; Muhammad Furqan Bari; Lubna Khan; Quratulain Amir; Armeen Ashraf; Ajia Ashraf; Zeba Haque; Mahrukh Qaiser; Farina Hanif. "Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs". سامانه مدیریت نشریات علمی, , , 1405, -. doi: 10.30476/mejc.2026.105034.2219
Ansari, Amir, Razzak, Zaid, Bari, Muhammad, Khan, Lubna, Amir, Quratulain, Ashraf, Armeen, Ashraf, Ajia, Haque, Zeba, Qaiser, Mahrukh, Hanif, Farina. (1405). 'Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/mejc.2026.105034.2219
Ansari, Amir, Razzak, Zaid, Bari, Muhammad, Khan, Lubna, Amir, Quratulain, Ashraf, Armeen, Ashraf, Ajia, Haque, Zeba, Qaiser, Mahrukh, Hanif, Farina. Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs. سامانه مدیریت نشریات علمی, 1405; (): -. doi: 10.30476/mejc.2026.105034.2219

Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs

Middle East Journal of Cancer
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 12 فروردین 1405    اصل مقاله (899.76 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2026.105034.2219
نویسندگان
Amir Ahmad Ansari1؛ Zaid Abdul Razzak2؛ Muhammad Furqan Bari3؛ Lubna Khan4؛ Quratulain Amir5؛ Armeen Ashraf6؛ Ajia Ashraf7؛ Zeba Haque8؛ Mahrukh Qaiser9؛ Farina Hanif* 10
1Department of Anatomy, Dow International Medical College, Dow University of Health Sciences
2Dr. Punjwani Center for Molecular Medicine and Drug Research, University of Karachi, Pakistan
3Department of Pathology, Dow International Medical College, Dow University of Health Sciences, Karachi, Pakistan
4Department of Biotechnology, University of Karachi, Karachi, Pakistan
5: Dow Institute of Medical Technology, Dow University of Health Sciences
6Department of Biochemistry, Dow International Medical College, Dow University of Health Sciences,
7Department of Biochemistry, Dow International Medical College, Dow University of Health Sciences
8Department of Anatomy, Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan
9Department of Anatomy, Dow Medical College, Dow University of Health Sciences
10Dow University of Health Sciences
چکیده
Background: Radiation therapy damages surrounding tissues, with parotid glands being highly vulnerable in the head and neck region. Antioxidant and anti-inflammatory properties of Vitamin C may reduce radiation-induced injury. This study therefore investigated the impact of vitamin C on morphological and histological changes in guinea pig parotid glands and its impact on Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-1 beta (IL-1β) expression.
Material and Methods: Forty-eight Dunkin Hartley guinea pigs (6-8 months, both sexes) were divided into four groups: control, Vitamin C (3 mg/kg), radiation 8 Grays (Gys), and radiation + Vitamin C. Animals were treated with radiation and/or Vitamin C for 15 days on alternate days. Weight and saliva volume were measured on alternate days too. Parotid glands were extracted for histological and immunohistochemical analysis of TNF-α and IL-1β. Data were analyzed using SPSS (version 21) and presented as mean ± SEM. One-way ANOVA with Bonferroni’s post hoc test was applied, with P < 0.05 considered significant.
Results: Over 15 days, no significant differences in animal weight were observed (P>0.05). However, a significant reduction in saliva production occurred in the radiation group, alleviated by Vitamin C (P<0.05). Histological analysis showed radiation-induced inflammation in the parotid glands, which was diminished by Vitamin C. Immunohistochemistry revealed a significant increase in TNF-α and IL-1β expression in the radiation group (P<0.001), with Vitamin C significantly downregulating both markers in the radiation + Vitamin C group (P<0.001).
Conclusion: Vitamin C may protect against radiation-induced parotid gland inflammation by downregulating TNF-α and IL-1β, suggesting its potential as a therapy for radiation-induced tissue injury.

تازه های تحقیق

Muhammad Furqan Bari (google scholar)

Zeba Haque (google scholar)

کلیدواژه‌ها
Parotid glands؛ Radiation؛ Vitamin C؛ TNF α and IL-1β
مراجع
  1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021;71:209-49. doi: 10.3322/caac.21660. PubMed PMID: 33538338.
  2. Johnson DE, Burtness B, Leemans CR, Lui VWY, Bauman JE, Grandis JR. Head and neck squamous cell carcinoma. Nat Rev Dis Primers. 2020;6:92. doi: 10.1038/s41572-020-00224-3. PubMed PMID: 33243986; PubMed Central PMCID: PMCPMC7944998.
  3. Thomas SJ, Penfold CM, Waylen A, Ness AR. The changing aetiology of head and neck squamous cell cancer: A tale of three cancers? Clin Otolaryngol. 2018;43:999-1003. doi: 10.1111/coa.13144. PubMed PMID: 29770611.
  4. Adelstein D, Gillison ML, Pfister DG, Spencer S, Adkins D, Brizel DM, et al. NCCN Guidelines Insights: Head and Neck Cancers, Version 2.2017. J Natl Compr Canc Netw. 2017;15:761-70. doi: 10.6004/jnccn.2017.0101. PubMed PMID: 28596256.
  5. Liu Z, Dong L, Zheng Z, Liu S, Gong S, Meng L, et al. Mechanism, Prevention, and Treatment of Radiation-Induced Salivary Gland Injury Related to Oxidative Stress. Antioxidants (Basel). 2021;10:1666. doi: 10.3390/antiox10111666. PubMed PMID: 34829539; PubMed Central PMCID: PMCPMC8614677.
  6. King SN, Dunlap NE, Tennant PA, Pitts T. Pathophysiology of Radiation-Induced Dysphagia in Head and Neck Cancer. Dysphagia. 2016;31:339-51. doi: 10.1007/s00455-016-9710-1. PubMed PMID: 27098922; PubMed Central PMCID: PMCPMC5340192.
  7. Sood AJ, Fox NF, O'Connell BP, Lovelace TL, Nguyen SA, Sharma AK, et al. Salivary gland transfer to prevent radiation-induced xerostomia: a systematic review and meta-analysis. Oral Oncol. 2014;50:77-83. doi: 10.1016/j.oraloncology.2013.10.010. PubMed PMID: 24189058.
  8. Nathan CO, Asarkar AA, Entezami P, Corry J, Strojan P, Poorten VV, et al. Current management of xerostomia in head and neck cancer patients. Am J Otolaryngol. 2023;44:103867. doi: 10.1016/j.amjoto.2023.103867. PubMed PMID: 36996514.
  9. Tribius S, Haladyn S, Hanken H, Busch CJ, Krull A, Petersen C, et al. Parotid sparing and quality of life in long-term survivors of locally advanced head and neck cancer after intensity-modulated radiation therapy. Strahlenther Onkol. 2021;197:219-30. doi: 10.1007/s00066-020-01737-2. PubMed PMID: 33377992.
  10. Szumiel I. Ionizing radiation-induced oxidative stress, epigenetic changes and genomic instability: the pivotal role of mitochondria. Int J Radiat Biol. 2015;91:1-12. doi: 10.3109/09553002.2014.934929. PubMed PMID: 24937368.
  11. Marmary Y, Adar R, Gaska S, Wygoda A, Maly A, Cohen J, et al. Radiation-induced loss of salivary gland function is driven by cellular senescence and prevented by IL6 modulation. 2016;76:1170-80.
  12. Yahyapour R, Amini P, Rezapour S, Cheki M, Rezaeyan A, Farhood B, et al. Radiation-induced inflammation and autoimmune diseases. Mil Med Res. 2018;5:9. doi: 10.1186/s40779-018-0156-7. PubMed PMID: 29554942; PubMed Central PMCID: PMCPMC5859747.
  13. Siva S, MacManus MP, Martin RF, Martin OAJCl. Abscopal effects of radiation therapy: a clinical review for the radiobiologist. 2015;356:82-90.
  14. Chambial S, Dwivedi S, Shukla KK, John PJ, Sharma P. Vitamin C in disease prevention and cure: an overview. Indian J Clin Biochem. 2013;28:314-28. doi: 10.1007/s12291-013-0375-3. PubMed PMID: 24426232; PubMed Central PMCID: PMCPMC3783921.
  15. Sato T, Kinoshita M, Yamamoto T, Ito M, Nishida T, Takeuchi M, et al. Treatment of irradiated mice with high-dose ascorbic acid reduced lethality. PLoS One. 2015;10:e0117020. doi: 10.1371/journal.pone.0117020. PubMed PMID: 25651298; PubMed Central PMCID: PMCPMC4317183.
  16. Saga R, Uchida T, Takino Y, Kondo Y, Kobayashi H, Kinoshita M, et al. Radiation-induced gastrointestinal syndrome is exacerbated in vitamin C–insufficient SMP30/GNL knockout mice. 2021;81:110931.
  17. Jafari E, Alavi M, Zal F. The evaluation of protective and mitigating effects of vitamin C against side effects induced by radioiodine therapy. Radiat Environ Biophys. 2018;57:233-40. doi: 10.1007/s00411-018-0744-7. PubMed PMID: 29860661.
  18. Jentzen W, Richter M, Nagarajah J, Poeppel TD, Brandau W, Dawes C, et al. Chewing-gum stimulation did not reduce the absorbed dose to salivary glands during radioiodine treatment of thyroid cancer as inferred from pre-therapy 124I PET/CT imaging. 2014;1:100.
  19. Auttara-Atthakorn A, Sungmala J, Anothaisintawee T, Reutrakul S, Sriphrapradang C. Prevention of salivary gland dysfunction in patients treated with radioiodine for differentiated thyroid cancer: A systematic review of randomized controlled trials. Front Endocrinol (Lausanne). 2022;13:960265. doi: 10.3389/fendo.2022.960265. PubMed PMID: 36105397; PubMed Central PMCID: PMCPMC9465079.
  20. Nair RP, Zheng C, Sunavala-Dossabhoy G. Retroductal Submandibular Gland Instillation and Localized Fractionated Irradiation in a Rat Model of Salivary Hypofunction. J Vis Exp. 2016:53785. doi: 10.3791/53785. PubMed PMID: 27168158; PubMed Central PMCID: PMCPMC4941994.
  21. Medicine WUSo LN. HEMATOXYLIN & EOSIN (H & E) STAIN PROTOCOL: Medicine WUSo, Lab N.; 1997 [cited 2023 March 22]. Available from: https://neuromuscular.wustl.edu/pathol/histol/HE.pdf.
  22. Jones J DF BD, et al. Parotid space: Radiopaedia.org; 2010 [cited 2023 March 22]. Available from: : https://radiopaedia.org/articles/parotid-space?lang=us.
  23. Pei X, Gu Q, Ye D, Wang Y, Zou X, He L, et al. Effect of computer radiation on weight and oxidant-antioxidant status of mice. 2015;31:1183-6.
  24. Zhang T, Liu C, Ma S, Gao Y, Wang R. Protective Effect and Mechanism of Action of Rosmarinic Acid on Radiation-Induced Parotid Gland Injury in Rats. Dose Response. 2020;18:1559325820907782. doi: 10.1177/1559325820907782. PubMed PMID: 32127788; PubMed Central PMCID: PMCPMC7036515.
  25. Hu L, Zhu Z, Hai B, Chang S, Ma L, Xu Y, et al. Intragland Shh gene delivery mitigated irradiation-induced hyposalivation in a miniature pig model. Theranostics. 2018;8:4321-31. doi: 10.7150/thno.26509. PubMed PMID: 30214623; PubMed Central PMCID: PMCPMC6134926.
  26. Lopez-Jornet P, Gómez-García F, Carrillo NG, Valle-Rodríguez E, Xerafin A, Vicente-Ortega VJBJoO, et al. Radioprotective effects of lycopene and curcumin during local irradiation of parotid glands in Sprague Dawley rats. 2016;54:275-9.
  27. Zhu Z, Pang B, Iglesias-Bartolome R, Wu X, Hu L, Zhang C, et al. Prevention of irradiation-induced salivary hypofunction by rapamycin in swine parotid glands. Oncotarget. 2016;7:20271-81. doi: 10.18632/oncotarget.7941. PubMed PMID: 26958808; PubMed Central PMCID: PMCPMC4991453.
  28. Ghoneim FM, Arafat EA. Histological and histochemical study of the protective role of rosemary extract against harmful effect of cell phone electromagnetic radiation on the parotid glands. Acta Histochem. 2016;118:478-85. doi: 10.1016/j.acthis.2016.04.010. PubMed PMID: 27155802.
  29. Wu VWC, Leung KY. A Review on the Assessment of Radiation Induced Salivary Gland Damage After Radiotherapy. Front Oncol. 2019;9:1090. doi: 10.3389/fonc.2019.01090. PubMed PMID: 31750235; PubMed Central PMCID: PMCPMC6843028.
  30. Mortazavi SMJ, Mortazavi G, Paknahad M. Histological and histochemical study of the protective role of rosemary extract against harmful effect of cell phone electromagnetic radiation on the parotid glands. Acta Histochem. 2016;118:657-8. doi: 10.1016/j.acthis.2016.07.004. PubMed PMID: 27439729.
  31. Astradsson T, Sellberg F, Berglund D, Ehrsson YT, Laurell GFEJFio. Systemic inflammatory reaction in patients with head and neck cancer—an explorative study. 2019;9:1177.
  32. Sun H, Cai H, Fu Y, Wang Q, Ji K, Du L, et al. The Protection Effect of Resveratrol Against Radiation-Induced Inflammatory Bowel Disease via NLRP-3 Inflammasome Repression in Mice. Dose Response. 2020;18:1559325820931292. doi: 10.1177/1559325820931292. PubMed PMID: 32636719; PubMed Central PMCID: PMCPMC7323307.
  33. Chen Y, Luo G, Yuan J, Wang Y, Yang X, Wang X, et al. Vitamin C mitigates oxidative stress and tumor necrosis factor‐alpha in severe community‐acquired pneumonia and LPS‐induced macrophages. 2014;2014:426740.
  34. Qing Z, Xiao-Hui W, Xi-Mei W, Chao-Chun Z. Vitamin C deficiency aggravates tumor necrosis factor alpha-induced insulin resistance. Eur J Pharmacol. 2018;829:1-11. doi: 10.1016/j.ejphar.2018.03.044. PubMed PMID: 29625084.
آمار
تعداد مشاهده مقاله: 18
تعداد دریافت فایل اصل مقاله: 7


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب