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Ansari, Amir, Razzak, Zaid, Bari, Muhammad, Khan, Lubna, Amir, Quratulain, Ashraf, Armeen, Kamran, Mahrukh, Ashraf, Ajia, Haque, Zeba, Hanif, Farina. (1405). Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs. سامانه مدیریت نشریات علمی, 17(2), 116-127. doi: 10.30476/mejc.2026.105034.2219
Amir Ahmad Ansari; Zaid Abdul Razzak; Muhammad Furqan Bari; Lubna Khan; Quratulain Amir; Armeen Ashraf; Mahrukh Kamran; Ajia Ashraf; Zeba Haque; Farina Hanif. "Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs". سامانه مدیریت نشریات علمی, 17, 2, 1405, 116-127. doi: 10.30476/mejc.2026.105034.2219
Ansari, Amir, Razzak, Zaid, Bari, Muhammad, Khan, Lubna, Amir, Quratulain, Ashraf, Armeen, Kamran, Mahrukh, Ashraf, Ajia, Haque, Zeba, Hanif, Farina. (1405). 'Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs', سامانه مدیریت نشریات علمی, 17(2), pp. 116-127. doi: 10.30476/mejc.2026.105034.2219
Ansari, Amir, Razzak, Zaid, Bari, Muhammad, Khan, Lubna, Amir, Quratulain, Ashraf, Armeen, Kamran, Mahrukh, Ashraf, Ajia, Haque, Zeba, Hanif, Farina. Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs. سامانه مدیریت نشریات علمی, 1405; 17(2): 116-127. doi: 10.30476/mejc.2026.105034.2219

Vitamin C Mitigates Radiation-Induced Parotid Glands Inflammation by Downregulating TNF-α and IL-1β Expression in Guinea Pigs

Middle East Journal of Cancer
دوره 17، شماره 2، تیر 2026، صفحه 116-127    اصل مقاله (4.05 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2026.105034.2219
نویسندگان
Amir Ahmad Ansari1؛ Zaid Abdul Razzak2؛ Muhammad Furqan Bari3؛ Lubna Khan4؛ Quratulain Amir5، 6؛ Armeen Ashraf7؛ Mahrukh Kamran1؛ Ajia Ashraf7؛ Zeba Haque8؛ Farina Hanif* 8
1Department of Anatomy, Dow International Medical College, Dow University of Health Sciences, Karachi, Pakistan
2Dr. Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Karachi, Pakistan
3Department of Pathology, Dow International Medical College, Dow University of Health Sciences, Karachi, Pakistan
4Department of Biotechnology, University of Karachi, Karachi, Pakistan
5Dow Institute of Medical Technology, Dow University of Health Sciences, Karachi, Pakistan
6Department of Physiology, University of Karachi, Karachi, Pakistan
7Dow International Medical College, Dow University of Health Sciences, Karachi, Pakistan
8Department of Biochemistry, Dow International Medical College, Dow University of Health Sciences, Karachi, Pakistan
چکیده
Background: Radiation therapy damages surrounding tissues, with parotid glands being highly vulnerable in the head and neck region. Antioxidant and anti-inflammatory properties of vitamin C may reduce radiation-induced injury. This study therefore investigated the impact of vitamin C on morphological and histological changes in guinea pig parotid glands and its impact on tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) expression.
Method: 48 Dunkin Hartley guinea pigs (6-8 months, both sexes) were divided into four groups: control, vitamin C (3 mg/kg), radiation 8 Grays (Gys), and radiation + vitamin C. Animals were treated with radiation and/or vitamin C for 15 days on alternate days. Weight and saliva volume were measured on alternate days too. Parotid glands were extracted for histological and immunohistochemical analysis of TNF-α and IL-1β. Data were analyzed using SPSS (version 21) and presented as mean ± standard error of mean. One-way ANOVA with Bonferroni’s post hoc test was applied, with P < 0.05 considered significant.
Results: Over 15 days, no significant differences in animal weight were observed (P > 0.05). However, a significant reduction in saliva production occurred in the radiation group, alleviated by vitamin C (P < 0.05). Histological analysis showed radiation-induced inflammation in the parotid glands, which was diminished by vitamin C. Immunohistochemistry revealed a significant increase in TNF-α and IL-1β expression in the radiation group (P < 0.001), with vitamin C significantly downregulating both markers in the radiation + vitamin C group (P < 0.001).
Conclusion: Vitamin C may protect against radiation-induced parotid gland inflammation by downregulating TNF-α and IL-1β, suggesting its potential as a therapy for radiation-induced tissue injury.

تازه های تحقیق

Muhammad Furqan Bari (google scholar)

Zeba Haque (google scholar)

کلیدواژه‌ها
Parotid glands؛ Radiation؛ Vitamin C؛ TNF α؛ IL-1β
مراجع
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