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Evaluation of the Synergistic Effects of Photobiomodulation and Natural Killer Cell Therapy in a Colorectal Cancer Xenograft Mouse Model | ||
| Iranian Journal of Colorectal Research | ||
| دوره 13، شماره 3، آذر 2025 اصل مقاله (2.9 M) | ||
| نوع مقاله: Research/Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/acrr.2025.109373.1276 | ||
| نویسندگان | ||
| najmeh malekzadeh Gonabdi1؛ hossein salehi-shadkami2؛ Mina yourtchi3؛ mehdi shamsara4؛ Dormohammad kordiTamandani1؛ mohammad vaezi3؛ sahar shojae5؛ Mehdi Alavi6؛ Mohammad Ahmadvand* 3 | ||
| 1Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran | ||
| 2Department of Medical Science, Tehran University of Medical Sciences, Tehran, Iran | ||
| 3Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Research Institute for Oncology, Hematology, and Cell | ||
| 4Department of Animal Biotechnology, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran | ||
| 5Middle East Gene and Cell Therapy Company, Tehran, Iran | ||
| 6Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran | ||
| چکیده | ||
| Background: Colorectal cancer (CRC) is a major cause of cancer-related mortality worldwide, highlighting the need for more effective therapeutic approaches. This study aimed to evaluate the synergistic antitumor effects of combining natural killer (NK) cell-based immunotherapy with photobiomodulation (PBM) in a CRC xenograft model. Methods: Human peripheral blood-derived NK cells were isolated, and their cytotoxic activity was confirmed in vitro against HT-29 CRC cells. Nude mice bearing HT-29 xenografts were assigned to four groups: control, PBM (808 nm), NK therapy, and combined NK + PBM treatment. Tumor size was monitored throughout the study, and excised tumors were analyzed histologically for NK cell infiltration, mitotic index, and apoptosis. Results: Both NK therapy and PBM alone significantly reduced tumor growth compared to the control. The combined NK + PBM group demonstrated the greatest suppression of tumor progression. Histopathological analysis revealed markedly enhanced NK cell infiltration, decreased mitotic activity, and increased apoptosis in the combination group compared to the monotherapy groups. Conclusion: NK therapy and PBM each exert anti-tumor effects in CRC xenografts; however, their combination produces a superior therapeutic response. PBM may serve as a promising adjuvant to enhance NK-based immunotherapy in CRC. | ||
| کلیدواژهها | ||
| Colorectal Neoplasms؛ Natural Killer Cells؛ Low-Level Light Therapy؛ Immunotherapy | ||
| مراجع | ||
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