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El-Far, Mohamed, Foda, Basma, Swellam, Menha, Abouzid, Amr, Abdelrazek, Mohamed. (1405). The Validation of Aberrant TM6SF1 Gene Methylation in the Diagnosis of Breast Cancer Patients. سامانه مدیریت نشریات علمی, 17(2), 106-115. doi: 10.30476/mejc.2025.104201.2169
Mohamed El-Far; Basma M. Foda; Menha Swellam; Amr Abouzid; Mohamed A. Abdelrazek. "The Validation of Aberrant TM6SF1 Gene Methylation in the Diagnosis of Breast Cancer Patients". سامانه مدیریت نشریات علمی, 17, 2, 1405, 106-115. doi: 10.30476/mejc.2025.104201.2169
El-Far, Mohamed, Foda, Basma, Swellam, Menha, Abouzid, Amr, Abdelrazek, Mohamed. (1405). 'The Validation of Aberrant TM6SF1 Gene Methylation in the Diagnosis of Breast Cancer Patients', سامانه مدیریت نشریات علمی, 17(2), pp. 106-115. doi: 10.30476/mejc.2025.104201.2169
El-Far, Mohamed, Foda, Basma, Swellam, Menha, Abouzid, Amr, Abdelrazek, Mohamed. The Validation of Aberrant TM6SF1 Gene Methylation in the Diagnosis of Breast Cancer Patients. سامانه مدیریت نشریات علمی, 1405; 17(2): 106-115. doi: 10.30476/mejc.2025.104201.2169

The Validation of Aberrant TM6SF1 Gene Methylation in the Diagnosis of Breast Cancer Patients

Middle East Journal of Cancer
دوره 17، شماره 2، تیر 2026، صفحه 106-115    اصل مقاله (2.11 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2025.104201.2169
نویسندگان
Mohamed El-Far* 1؛ Basma M. Foda1؛ Menha Swellam2؛ Amr Abouzid3؛ Mohamed A. Abdelrazek4
1Chemistry Department, Biochemistry Division, Faculty of Science, Mansoura University, Mansoura, Egypt
2Biochemistry Department, Biotechnology Research Institute, High Throughput Molecular and Genetic Laboratory, Central Laboratories Network and the Centers of Excellence, National Research Centre, Dokki, Giza, Egypt
3Department of Surgical Oncology, Mansoura Oncology Centre, Faculty of Medicine, Mansoura University, Mansoura, Egypt
4Research and Development Department, Biotechnology Research Center, New Damietta, Egypt
چکیده
Background: The limitations of breast cancer (BC) diagnosis and prognosis strategies are well-established. The present study aimed to evaluate transmembrane 6 superfamily member 1 (TM6SF1) DNA methylation as non-invasive biomarker for differentiating BC from non-cancer individuals (with benign breast diseases and healthy controls). Also, this study aimed to evaluate the association between TM6SF1 methylation and clinicopathological BC features.
Method: A total of 200 women were enrolled in this retrospective study. The study participants were divided into three groups of 120 women with primary BC, 40 women with benign breast diseases, and 40 normal healthy controls. Blood sample were withdrawn and DNA was extracted. Then, TM6SF1 DNA methylation level was detected using a quantitative polymerase chain reaction assay. Data were analyzed using GraphPad prism (version 6) and SPSS (version 20) programs. The statistical differences between the three groups were determined using ANOVA and Kruskal-Wallis tests. A P value < 0.05 was considered to be statistically significant.
Results: The study findings revealed that hyper methylation of TM6SF1 level was reported higher in BC patients [82.2 (56.8-87.7)] as compared with benign [28.9 (27.9-64.8)] or healthy [27.8 (13-28.8)] controls at P = 0.0001. TM6SF1 methylation had superior diagnostic efficacy as compared with established BC markers [carcinoembryonic antigen and cancer antigen (CA15.3)]. Also, methylation level showed significant difference among clinical stages, histological grading and lymph-node invasion.
Conclusion: The present study reveals that aberrant TM6SF1 methylation is associated with BC tumorgenesity. It could facilitate early BC detection from benign breast diseases. Hypermethylated TM6SF1 is associated with disease progression suggesting its potential role as diagnostic and prognostic biomarker for BC.

تازه های تحقیق

Mohamed A. Abdelrazek (googl escholar)

 

کلیدواژه‌ها
Breast cancer؛ Early diagnosis؛ TM6SF1؛ DNA methylation؛ Circulating molecular markers
مراجع
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