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Lin, Yu, Huang, NengPing, He, YuanBin, Zuo, PengJu, Yang, LiQiu, Huang, FengJia, Ye, YongQing. (1404). In Vitro Expansion of Dendritic Cells Pulsed with Placental Peptides for the Generation of Antigen-Specific T Cells with Antitumor Activity. سامانه مدیریت نشریات علمی, 22(4), -. doi: 10.22034/iji.2025.107705.3075
Yu Lin; NengPing Huang; YuanBin He; PengJu Zuo; LiQiu Yang; FengJia Huang; YongQing Ye. "In Vitro Expansion of Dendritic Cells Pulsed with Placental Peptides for the Generation of Antigen-Specific T Cells with Antitumor Activity". سامانه مدیریت نشریات علمی, 22, 4, 1404, -. doi: 10.22034/iji.2025.107705.3075
Lin, Yu, Huang, NengPing, He, YuanBin, Zuo, PengJu, Yang, LiQiu, Huang, FengJia, Ye, YongQing. (1404). 'In Vitro Expansion of Dendritic Cells Pulsed with Placental Peptides for the Generation of Antigen-Specific T Cells with Antitumor Activity', سامانه مدیریت نشریات علمی, 22(4), pp. -. doi: 10.22034/iji.2025.107705.3075
Lin, Yu, Huang, NengPing, He, YuanBin, Zuo, PengJu, Yang, LiQiu, Huang, FengJia, Ye, YongQing. In Vitro Expansion of Dendritic Cells Pulsed with Placental Peptides for the Generation of Antigen-Specific T Cells with Antitumor Activity. سامانه مدیریت نشریات علمی, 1404; 22(4): -. doi: 10.22034/iji.2025.107705.3075

In Vitro Expansion of Dendritic Cells Pulsed with Placental Peptides for the Generation of Antigen-Specific T Cells with Antitumor Activity

Iranian Journal of Immunology
دوره 22، شماره 4، اسفند 2025
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2025.107705.3075
نویسندگان
Yu Lin1؛ NengPing Huang2؛ YuanBin He1؛ PengJu Zuo2؛ LiQiu Yang2؛ FengJia Huang2؛ YongQing Ye* 2
1Department of Pediatric Surgery, Fujian Children's Hospital (Fujian Branch of Shanghai Children's Medical Center), College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University 350001 Fujian Province, China.
2Xiamen Stemery Biotech Co., Ltd Bld. 5#, Haican Biobay, 2002 Wengjiao Road, Xiamen, Fujian Province, China.
چکیده
Background: Placental tissue contains a variety of tumor-associated antigens. Antigen-specific T cells generated by expanded dendritic cells (DCs) loaded with placental peptides have the potential to exert antitumor effects both in vitro and in vivo.
Objective: To investigate the immunotherapeutic potential of placental peptides as a novel source of tumor-associated antigens (TAAs). We hypothesized that DCs, expanded and matured in vitro and pulsed with placental peptide extracts, can effectively prime antigen-specific T cells (ASTs) with robust cytotoxic activity against various tumor cell lines and in vivo tumor models.
Methods: Mass spectrometry was used to identify tumor-related peptides within the placental extract. In vitro assays were employed to assess the expansion of DCs, their maturation following exposure to placental peptide preparations, and the subsequent activation of T cells.
Results: The cytotoxic activity of ASTs was assessed and showed strong tumor cell killing across three cancer cell lines, U87MG (glioblastoma), SH-SY5Y (neuroblastoma), and MCF-7 (breast cancer). In a neuroblastoma animal model, ASTs treatment significantly reduced tumor proliferation, indicating substantial therapeutic potential in vivo.
Conclusion: Our findings suggest that DCs pulsed with placental peptides can generate ASTs with potent antitumor activity in vitro and in vivo. Additional studies are needed to determine applicability across other tumor types, refine therapeutic parameters, and assess clinical potential.
کلیدواژه‌ها
Antigen-specific T cells؛ Cancer immunotherapy, Dendritic cell expansion؛ Neuroblastoma, Placental peptides
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