LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,242
تعداد مقالات11,287
تعداد مشاهده مقاله80,560,812
تعداد دریافت فایل اصل مقاله116,599,718
Li, Chenxi, Lin, Mingxin, Zhu, Min, Lin, Jianmin, Li, Xiujuan, Ye, Huiming. (1404). Differential Functions of Membrane-bound and Soluble TNF-α in Regulating Trophoblasts Invasion and Migration. سامانه مدیریت نشریات علمی, 22(4), -. doi: 10.22034/iji.2025.106019.3011
Chenxi Li; Mingxin Lin; Min Zhu; Jianmin Lin; Xiujuan Li; Huiming Ye. "Differential Functions of Membrane-bound and Soluble TNF-α in Regulating Trophoblasts Invasion and Migration". سامانه مدیریت نشریات علمی, 22, 4, 1404, -. doi: 10.22034/iji.2025.106019.3011
Li, Chenxi, Lin, Mingxin, Zhu, Min, Lin, Jianmin, Li, Xiujuan, Ye, Huiming. (1404). 'Differential Functions of Membrane-bound and Soluble TNF-α in Regulating Trophoblasts Invasion and Migration', سامانه مدیریت نشریات علمی, 22(4), pp. -. doi: 10.22034/iji.2025.106019.3011
Li, Chenxi, Lin, Mingxin, Zhu, Min, Lin, Jianmin, Li, Xiujuan, Ye, Huiming. Differential Functions of Membrane-bound and Soluble TNF-α in Regulating Trophoblasts Invasion and Migration. سامانه مدیریت نشریات علمی, 1404; 22(4): -. doi: 10.22034/iji.2025.106019.3011

Differential Functions of Membrane-bound and Soluble TNF-α in Regulating Trophoblasts Invasion and Migration

Iranian Journal of Immunology
دوره 22، شماره 4، اسفند 2025
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2025.106019.3011
نویسندگان
Chenxi Li؛ Mingxin Lin؛ Min Zhu؛ Jianmin Lin؛ Xiujuan Li* ؛ Huiming Ye*
Department of Laboratory Medicine, Fujian Key Clinical Specialty of Laboratory Medicine, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China.
چکیده
Background: Proper invasion and migration of trophoblasts are critical for successful human pregnancy. Tumor necrosis factor-α (TNF-α) has two biologically active forms: the membrane-bound TNF-α (mTNF) and the soluble TNF-α (sTNF).
Objective: To investigate the role of both forms of TNF-α in trophoblasts invasion and migration and to elucidate their underlying mechanisms.
Methods: We exposed HTR-8/SVneo trophoblasts to exogenous mTNF or sTNF and evaluated their cellular behaviors. Proliferation was assessed using a Cell Counting Kit-8, while invasion and migration were assessed through Transwell assays. Additionally, we measured mRNA levels of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinases-1 (TIMP-1), and plasminogen activator inhibitor-1 (PAI-1) using qRT-PCR. The expression of IκB-α was determined by western blot analysis.
Results: Unlike sTNF, mTNF enhances the invasion and migration of trophoblasts. Mechanistic analysis showed that mTNF increased MMP-9 expression while decreasing TIMP-1 and PAI-1 expression, and it inhibited the activation of NF-κB signaling pathway in HTR-8/SVneo cells with or without lipopolysaccharide (LPS) treatment.
Conclusion: This research uncovered a new function of mTNF in regulating trophoblast invasion and migration, offering a new approach to treating pregnancy-related diseases associated with inadequate trophoblast invasion.
کلیدواژه‌ها
Invasion؛ Membrane-bound TNF-α؛ Migration؛ NF-κB signaling pathway؛ Trophoblast
آمار
تعداد مشاهده مقاله: 13


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب