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Ilyas, Syafruddin, Situmorang, Putri Cahaya, Ahmad, Hafandi, Khairani, Dina, Wati, Dini Prastyo, Polem, Wardah Sawitri. (1404). Exploring Nanoherbal Paraboea leuserensis as a Therapeutic Agent in Traumatic Brain Injury: In Silico, and In Vivo Approaches. سامانه مدیریت نشریات علمی, 50(12), 819-831. doi: 10.30476/ijms.2025.106167.4027
Syafruddin Ilyas; Putri Cahaya Situmorang; Hafandi Ahmad; Dina Khairani; Dini Prastyo Wati; Wardah Sawitri Polem. "Exploring Nanoherbal Paraboea leuserensis as a Therapeutic Agent in Traumatic Brain Injury: In Silico, and In Vivo Approaches". سامانه مدیریت نشریات علمی, 50, 12, 1404, 819-831. doi: 10.30476/ijms.2025.106167.4027
Ilyas, Syafruddin, Situmorang, Putri Cahaya, Ahmad, Hafandi, Khairani, Dina, Wati, Dini Prastyo, Polem, Wardah Sawitri. (1404). 'Exploring Nanoherbal Paraboea leuserensis as a Therapeutic Agent in Traumatic Brain Injury: In Silico, and In Vivo Approaches', سامانه مدیریت نشریات علمی, 50(12), pp. 819-831. doi: 10.30476/ijms.2025.106167.4027
Ilyas, Syafruddin, Situmorang, Putri Cahaya, Ahmad, Hafandi, Khairani, Dina, Wati, Dini Prastyo, Polem, Wardah Sawitri. Exploring Nanoherbal Paraboea leuserensis as a Therapeutic Agent in Traumatic Brain Injury: In Silico, and In Vivo Approaches. سامانه مدیریت نشریات علمی, 1404; 50(12): 819-831. doi: 10.30476/ijms.2025.106167.4027

Exploring Nanoherbal Paraboea leuserensis as a Therapeutic Agent in Traumatic Brain Injury: In Silico, and In Vivo Approaches

Iranian Journal of Medical Sciences
مقاله 2، دوره 50، شماره 12، اسفند 2025، صفحه 819-831    اصل مقاله (1.45 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2025.106167.4027
نویسندگان
Syafruddin Ilyas* 1؛ Putri Cahaya Situmorang1؛ Hafandi Ahmad2؛ Dina Khairani1؛ Dini Prastyo Wati1؛ Wardah Sawitri Polem1
1Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
2Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, University of Putra Malaysia, Selangor, Malaysia
چکیده
Background: Traumatic brain injury (TBI) is a major global health burden and one of the leading causes of death and disability worldwide, affecting up to 74 million people annually. It profoundly impairs mental health, quality of life, and daily functioning. This study aimed to explore the therapeutic potential of nanoherbal compounds from Paraboea leuserensis using combined in silico and in vivo approaches in a rat model of TBI.
Methods: In the in silico phase, bioactive compounds from Paraboea leuserensis leaves identified by Gas Chromatography–Mass Spectrometry (GC-MS) were screened through molecular docking to assess their binding affinity and pharmacokinetic properties. For the in vivo study, 30 male Wistar rats were allocated into six groups: G0 (normal control), G+(TBI control), MP (TBI+methylprednisolone 30 mg/Kg BW), and treatment groups PL100, PL200, and PL300 (TBI+nanoherbal extract at 100, 200, and 300 mg/Kg BW, respectively). Antioxidant activity was evaluated through superoxide dismutase (SOD) and malondialdehyde (MDA) assays. Data were analyzed by one-way ANOVA with Tukey’s post hoc test (P<0.05) using GraphPad Prism.
Results: GC-MS analysis revealed bioactive compounds with favorable pharmacokinetic properties. Molecular docking showed strong interactions of 9-octadecen-12-ynoic acid methyl ester with ERK2 and CCR2, while 9-octadecenoic acid (Z) displayed notable binding to JNK3. In vivo, PL100 (P<0.01), PL200 (P<0.001), and PL300 (P<0.0001) significantly enhanced SOD activity and reduced MDA levels compared to the TBI control.
Conclusion: Both in silico and in vivo findings highlight the neuroprotective potential of Paraboea leuserensis, with PL300 showing the most pronounced antioxidant effect in TBI-induced rats.

تازه های تحقیق

Syafruddin Ilyas (Google Scholar)

کلیدواژه‌ها
Traumatic brain injury؛ Phytotherapy؛ Molecular docking simulation؛ Oxidative stress؛ Superoxide dismutase
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