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Sokouti Nasimi, Fatemeh, Alimohammadzadeh, Behrad, Mehdizadeh, Amir, AL-Koofee, Dhafer A.F., Tamadon, Amin, Mahdipour, Mahdi, Rahbarghazi, Reza. (1404). Oxidative Stress Influences Exosome Biogenesis by Inducing Cellular Senescence in Uterine Adenocarcinoma Ishikawa Cells. سامانه مدیریت نشریات علمی, 50(12), 832-842. doi: 10.30476/ijms.2025.105914.3995
Fatemeh Sokouti Nasimi; Behrad Alimohammadzadeh; Amir Mehdizadeh; Dhafer A.F. AL-Koofee; Amin Tamadon; Mahdi Mahdipour; Reza Rahbarghazi. "Oxidative Stress Influences Exosome Biogenesis by Inducing Cellular Senescence in Uterine Adenocarcinoma Ishikawa Cells". سامانه مدیریت نشریات علمی, 50, 12, 1404, 832-842. doi: 10.30476/ijms.2025.105914.3995
Sokouti Nasimi, Fatemeh, Alimohammadzadeh, Behrad, Mehdizadeh, Amir, AL-Koofee, Dhafer A.F., Tamadon, Amin, Mahdipour, Mahdi, Rahbarghazi, Reza. (1404). 'Oxidative Stress Influences Exosome Biogenesis by Inducing Cellular Senescence in Uterine Adenocarcinoma Ishikawa Cells', سامانه مدیریت نشریات علمی, 50(12), pp. 832-842. doi: 10.30476/ijms.2025.105914.3995
Sokouti Nasimi, Fatemeh, Alimohammadzadeh, Behrad, Mehdizadeh, Amir, AL-Koofee, Dhafer A.F., Tamadon, Amin, Mahdipour, Mahdi, Rahbarghazi, Reza. Oxidative Stress Influences Exosome Biogenesis by Inducing Cellular Senescence in Uterine Adenocarcinoma Ishikawa Cells. سامانه مدیریت نشریات علمی, 1404; 50(12): 832-842. doi: 10.30476/ijms.2025.105914.3995

Oxidative Stress Influences Exosome Biogenesis by Inducing Cellular Senescence in Uterine Adenocarcinoma Ishikawa Cells

Iranian Journal of Medical Sciences
مقاله 3، دوره 50، شماره 12، اسفند 2025، صفحه 832-842    اصل مقاله (2.43 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2025.105914.3995
نویسندگان
Fatemeh Sokouti Nasimi1؛ Behrad Alimohammadzadeh1؛ Amir Mehdizadeh2؛ Dhafer A.F. AL-Koofee3؛ Amin Tamadon4؛ Mahdi Mahdipour1، 5؛ Reza Rahbarghazi* 1، 6
1Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Clinical Laboratory Science, Faculty of Pharmacy, University of Kufa, Najaf, Kufa 54003, Iraq
4Department of Natural Sciences, West-Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
5Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
6Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Background: The progression of life and cellular senescence can alter the physiological activity of every cell type. Here, the possible effect of oxidative stress on exosome (Exo) biogenesis was studied in endometrial adenocarcinoma Ishikawa cells. 
Methods: This in vitro study was conducted from 2022 to 2023 at the Stem Cell Research Center affiliated with Tabriz University of Medical Sciences. Cells were treated with 20 μM hydrogen peroxide (H2O2) for 4 days, and physicochemical properties of Exos were analyzed using dynamic light scattering (DLS), scanning electron microscope (SEM), and western blotting. The expression of genes such as ALIX, CD63, TSG101, Rab27a, and Rab27b, along with aging factor senescence-associated 𝛽-galactosidase (SA-β-gal), was studied using real-time PCR analysis. The fatty acid profile was determined in isolated Exos using gas chromatography. We also measured the exosomal content of superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA).  
Results: The expression of SA-β-gal confirmed the successful induction of aging in Ishikawa cells after 4 days (P=0.0286). DLS analysis indicated a slight increase and decrease in mean Exo size and zeta potential, respectively, in H2O2-treated Exos compared to the control group. Proteomic analysis revealed the lack of changes in exosomal levels of CD63 and CD81 tetraspanins in both groups (P=0.001). Real-time PCR analysis indicated the upregulation of ALIX and TSG101, while the expression of CD63 and Rab27b was reduced in H2O2-treated cells compared to the control group (P=0.0015 and P=0.0129). No statistically significant changes were found in exosomal levels of SOD, GPx, and MDA before and after treatment with the H2O2 (P=0.857, P=0.421, and P=0.3739). Data indicated an increase in exosomal polyunsaturated fatty acids and monounsaturated fatty acids in H2O2-treated cells compared to the control cells.
Conclusion: Oxidative stress can influence Exo biogenesis and paracrine activity in endometrial tumor cells via the induction of cellular senescence.

تازه های تحقیق

Fatemeh Sokouti Nasimi (Google Scholar)

Reza Rahbarghazi (Google Scholar)

کلیدواژه‌ها
Uterine neoplasms؛ Cellular senescence؛ Extracellular vesicles؛ Biogenesis؛ Paracrine communication
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