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Khoshandam, Mohadeseh, Soheili, Zahra-Soheila, Hosseinkhani, Saman, Samiee, Shahram, Latifi-Navid, Hamid, Kalhor, Naser, Soltaninejad, Hossein. (1404). Leveraging the htsFLT01/MiRGD Complex to Enhance Apoptosis and Suppress Angiogenesis in MCF7 Breast Cancer Cells. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/ijms.2025.105176.3884
Mohadeseh Khoshandam; Zahra-Soheila Soheili; Saman Hosseinkhani; Shahram Samiee; Hamid Latifi-Navid; Naser Kalhor; Hossein Soltaninejad. "Leveraging the htsFLT01/MiRGD Complex to Enhance Apoptosis and Suppress Angiogenesis in MCF7 Breast Cancer Cells". سامانه مدیریت نشریات علمی, , , 1404, -. doi: 10.30476/ijms.2025.105176.3884
Khoshandam, Mohadeseh, Soheili, Zahra-Soheila, Hosseinkhani, Saman, Samiee, Shahram, Latifi-Navid, Hamid, Kalhor, Naser, Soltaninejad, Hossein. (1404). 'Leveraging the htsFLT01/MiRGD Complex to Enhance Apoptosis and Suppress Angiogenesis in MCF7 Breast Cancer Cells', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/ijms.2025.105176.3884
Khoshandam, Mohadeseh, Soheili, Zahra-Soheila, Hosseinkhani, Saman, Samiee, Shahram, Latifi-Navid, Hamid, Kalhor, Naser, Soltaninejad, Hossein. Leveraging the htsFLT01/MiRGD Complex to Enhance Apoptosis and Suppress Angiogenesis in MCF7 Breast Cancer Cells. سامانه مدیریت نشریات علمی, 1404; (): -. doi: 10.30476/ijms.2025.105176.3884

Leveraging the htsFLT01/MiRGD Complex to Enhance Apoptosis and Suppress Angiogenesis in MCF7 Breast Cancer Cells

Iranian Journal of Medical Sciences
مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 24 شهریور 1404    اصل مقاله (394.92 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2025.105176.3884
نویسندگان
Mohadeseh Khoshandam1؛ Zahra-Soheila Soheili* 1؛ Saman Hosseinkhani2؛ Shahram Samiee3؛ Hamid Latifi-Navid1؛ Naser Kalhor4؛ Hossein Soltaninejad5
1Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
2Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
3Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
4Department of Cell Biology and Regenerative Medicine, Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran
5Department of stem cells technology and Tissue Regeneration, Faculty of Interdisciplinary Science and Technologies, Tarbiat Modares University, Tehran, Iran
چکیده
Background: Gene therapy introduces therapeutic genes into cancer cells to inhibit tumor growth or induce apoptosis. The htsFLT01 gene, a novel anti-angiogenic construct, encodes the sFLT01 protein that functions as a Vascular Endothelial Growth Factor (VEGF) decoy receptor, impeding pathological angiogenesis. When combined with the MiRGD nanocarrier—a versatile peptide-based delivery system optimized for specificity, biocompatibility, and low toxicity—the htsFLT01/MiRGD complex offers a potent strategy against breast cancer.
Methods: The htsFLT01 gene was designed and constructed in previous studies. The MiRGD peptide was expressed and purified using Ni-NTA affinity chromatography in the E. coli C41 (DE3) expression strain. The potency of this peptide, along with the cell viability and toxicity of the nanoparticles, was previously evaluated in MCF7 cell culture. After transfection with the htsFLT01/MiRGD nanocomplex at a nitrogen-to-phosphorus (N/P) ratio of 14, cell lysates were collected, and expression analysis of the key genes, including Fas-Associated Death Domain Protein (FADD), Caspase-8 (CASP8), and Tumor Protein P53 (TP53), was conducted based on findings from prior research. Statistical analyses were conducted using IBM SPSS Statistics version 22 (IBM, USA) and REST 2009 software.  
Results: The htsFLT01 gene was previously designed and constructed, and the MiRGD nanocarrier was successfully produced and purified. This nanocarrier exhibited the best performance at an N/P ratio of 14. This study evaluated the effect of this complex on apoptosis induction in MCF7 cells via the extrinsic apoptotic pathway, revealing increased expression of FADD, CASP8, and p53 genes. 
Conclusion: These findings highlight a synergistic relationship between anti-angiogenic and pro-apoptotic mechanisms, offering promising avenues for future breast cancer therapies.

تازه های تحقیق

Mohadeseh Khoshandam (Google Scholar)

Zahra-Soheila Soheili (Google Scholar)

کلیدواژه‌ها
CASP8 and FADD-like apoptosis regulating protein؛ Tumor suppressor protein p53؛ Breast neoplasms؛ Apoptosis؛ Angiogenesis inhibitors
مراجع
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