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Mousavikia, Seyedeh Nasibeh, M. Matin, Maryam, Bahreyni-Toossi, Mohammad Taghi, Aghaee-Bakhtiari, Seyed Hamid, Azimian, Hosein. (1404). Investigating miR-16-5p: The Tumor Suppressor That Influences Cell Cycle Genes in Colorectal Cancer. سامانه مدیریت نشریات علمی, 15(5), 451-458. doi: 10.31661/jbpe.v0i0.2501-1874
Seyedeh Nasibeh Mousavikia; Maryam M. Matin; Mohammad Taghi Bahreyni-Toossi; Seyed Hamid Aghaee-Bakhtiari; Hosein Azimian. "Investigating miR-16-5p: The Tumor Suppressor That Influences Cell Cycle Genes in Colorectal Cancer". سامانه مدیریت نشریات علمی, 15, 5, 1404, 451-458. doi: 10.31661/jbpe.v0i0.2501-1874
Mousavikia, Seyedeh Nasibeh, M. Matin, Maryam, Bahreyni-Toossi, Mohammad Taghi, Aghaee-Bakhtiari, Seyed Hamid, Azimian, Hosein. (1404). 'Investigating miR-16-5p: The Tumor Suppressor That Influences Cell Cycle Genes in Colorectal Cancer', سامانه مدیریت نشریات علمی, 15(5), pp. 451-458. doi: 10.31661/jbpe.v0i0.2501-1874
Mousavikia, Seyedeh Nasibeh, M. Matin, Maryam, Bahreyni-Toossi, Mohammad Taghi, Aghaee-Bakhtiari, Seyed Hamid, Azimian, Hosein. Investigating miR-16-5p: The Tumor Suppressor That Influences Cell Cycle Genes in Colorectal Cancer. سامانه مدیریت نشریات علمی, 1404; 15(5): 451-458. doi: 10.31661/jbpe.v0i0.2501-1874

Investigating miR-16-5p: The Tumor Suppressor That Influences Cell Cycle Genes in Colorectal Cancer

Journal of Biomedical Physics and Engineering
دوره 15، شماره 5 - شماره پیاپی 1، دی 2025، صفحه 451-458    اصل مقاله (1.18 M)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v0i0.2501-1874
نویسندگان
Seyedeh Nasibeh Mousavikia1، 2؛ Maryam M. Matin3، 4؛ Mohammad Taghi Bahreyni-Toossi2؛ Seyed Hamid Aghaee-Bakhtiari5، 6؛ Hosein Azimian* 1، 2
1Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Medical Physics Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
4Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
5Bioinformatics Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
6Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Background: Colorectal Cancer (CRC) remains a major health challenge, with perturbation in the cell cycle playing a crucial role in its progression. Hsa-miR-16-5p (miR-16-5p) is a crucial tumor suppressor, but its precise role in modulating cell cycle genes, particularly in the presence of ionizing radiation, is not fully understood.
Objective: This study investigated the role of miR-16-5p in modulating the expression of cell cycle genes in colorectal cancer cells exposed to ionizing radiation.
Material and Methods: In this experimental study, HT-29 cells were transfected with miR-16-5p using the polyfectamine transfection reagent. Expression levels of miR-16-5p, Cyclin-Dependent Kinase 4 (CDK4), Cyclin E1 (CCNE1), and Cyclin D1 (CCND1) were quantified by real-time Polymerase Chain Reaction (PCR). To assess the changes after irradiation, cells were exposed to 4 Gy.
Results: Ionizing radiation significantly downregulated miR-16-5p compared to controls, while transfection of miR-16-5p significantly increased its expression level. However, irradiation of 4 Gy did not significantly alter CCND1 or CCNE1, but decreased CDK4 expression. The miR-16-5p transfection significantly suppressed CCND1, CCNE1 and CDK4 compared to controls. The expression of CCND1, CCNE1, and CDK4 significantly decreased when miR-16-5p transfection was performed before 4 Gy irradiation compared to both 4 Gy irradiation alone and the control group. 
Conclusion: Our results highlight the role of miR-16-5p in modulating key cell cycle genes in CRC. Increasing miR-16-5p expression could improve radiosensitivity and represent a therapeutic strategy for the treatment of CRC.

تازه های تحقیق

Seyedeh Nasibeh Mousavikia (Google Scholar)

Hosein Azimian (Google Scholar)

کلیدواژه‌ها
MicroRNAs؛ Cell Cycle؛ Radiation-Sensitizing Agents؛ Colorectal Cancer
مراجع
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