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Zarshenas, Mohammad M., Nemati, Elham, Mousavi, Seyed Hossein, Rezaeifar, Niloofar, Khademian, Sedigheh, Morowvat, Mohammad Hossein. (1404). Molecular Identification and Phylogenetic Differentiation of Iranian Hypericum Species Using 18S rRNA Gene Barcoding and Bioinformatic Analysis. سامانه مدیریت نشریات علمی, 11(2), -. doi: 10.30476/tips.2025.107433.1304
Mohammad M. Zarshenas; Elham Nemati; Seyed Hossein Mousavi; Niloofar Rezaeifar; Sedigheh Khademian; Mohammad Hossein Morowvat. "Molecular Identification and Phylogenetic Differentiation of Iranian Hypericum Species Using 18S rRNA Gene Barcoding and Bioinformatic Analysis". سامانه مدیریت نشریات علمی, 11, 2, 1404, -. doi: 10.30476/tips.2025.107433.1304
Zarshenas, Mohammad M., Nemati, Elham, Mousavi, Seyed Hossein, Rezaeifar, Niloofar, Khademian, Sedigheh, Morowvat, Mohammad Hossein. (1404). 'Molecular Identification and Phylogenetic Differentiation of Iranian Hypericum Species Using 18S rRNA Gene Barcoding and Bioinformatic Analysis', سامانه مدیریت نشریات علمی, 11(2), pp. -. doi: 10.30476/tips.2025.107433.1304
Zarshenas, Mohammad M., Nemati, Elham, Mousavi, Seyed Hossein, Rezaeifar, Niloofar, Khademian, Sedigheh, Morowvat, Mohammad Hossein. Molecular Identification and Phylogenetic Differentiation of Iranian Hypericum Species Using 18S rRNA Gene Barcoding and Bioinformatic Analysis. سامانه مدیریت نشریات علمی, 1404; 11(2): -. doi: 10.30476/tips.2025.107433.1304

Molecular Identification and Phylogenetic Differentiation of Iranian Hypericum Species Using 18S rRNA Gene Barcoding and Bioinformatic Analysis

Trends in Pharmaceutical Sciences and Technologies
مقاله 7، دوره 11، شماره 2، شهریور 2025
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/tips.2025.107433.1304
نویسندگان
Mohammad M. Zarshenas1؛ Elham Nemati2؛ Seyed Hossein Mousavi3؛ Niloofar Rezaeifar3؛ Sedigheh Khademian3؛ Mohammad Hossein Morowvat* 4
1Department of phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. Essence of Parsiyan Wisdom Institute, Traditional Medicine and Medicinal Plant Incubator, Shiraz University
2Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
4Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 7146864685, Shiraz, Iran
چکیده
The Hypericum genus is renowned for its medicinal properties, yet its commercial products are often adulterated due to challenges in morphological identification. This study presents a molecular approach using 18S rRNA gene sequencing to identify and differentiate 12 Hypericum species collected from various regions of Iran. Genomic DNA was extracted, and the 18S rRNA ribosomal genes were amplified using specific primers, and sequenced. Besides, the morphologic characteristics of each sample were evaluated using light microscopy method. Bioinformatic analyses, including multiple sequence alignment, conserved domains shading and phylogenetic tree construction, revealed distinct clades corresponding to individual species, validating the efficiency of DNA barcoding of the studied Hypericum samples. This method offers a rapid, reliable, and reproducible tool for accurate species identification, which is essential for quality control in the herbal industry. The developed molecular identification methods can also be applied, with slight modifications, to identify other Hypericum specimens and similar plant species.
کلیدواژه‌ها
18S rRNA؛ DNA barcoding؛ Herbal authentication؛ Hypericum؛ Phylogenetic analysis
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