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Ghozali, Mohammad, Safitri, Ratu, Khristian, Erick, Ainni, Amethyst Puspita, Kuntana, Yasmi Purnamasari, Bashari, Muhamad Hasan, Purnama, Jeri Nobia. (1404). Impact of Sappan Wood Extract as Iron Chelator Adjuvant on Iron Concentration and Macrophage Polarization in Rat Spleen. سامانه مدیریت نشریات علمی, 50(7), 481-491. doi: 10.30476/ijms.2024.103099.3629
Mohammad Ghozali; Ratu Safitri; Erick Khristian; Amethyst Puspita Ainni; Yasmi Purnamasari Kuntana; Muhamad Hasan Bashari; Jeri Nobia Purnama. "Impact of Sappan Wood Extract as Iron Chelator Adjuvant on Iron Concentration and Macrophage Polarization in Rat Spleen". سامانه مدیریت نشریات علمی, 50, 7, 1404, 481-491. doi: 10.30476/ijms.2024.103099.3629
Ghozali, Mohammad, Safitri, Ratu, Khristian, Erick, Ainni, Amethyst Puspita, Kuntana, Yasmi Purnamasari, Bashari, Muhamad Hasan, Purnama, Jeri Nobia. (1404). 'Impact of Sappan Wood Extract as Iron Chelator Adjuvant on Iron Concentration and Macrophage Polarization in Rat Spleen', سامانه مدیریت نشریات علمی, 50(7), pp. 481-491. doi: 10.30476/ijms.2024.103099.3629
Ghozali, Mohammad, Safitri, Ratu, Khristian, Erick, Ainni, Amethyst Puspita, Kuntana, Yasmi Purnamasari, Bashari, Muhamad Hasan, Purnama, Jeri Nobia. Impact of Sappan Wood Extract as Iron Chelator Adjuvant on Iron Concentration and Macrophage Polarization in Rat Spleen. سامانه مدیریت نشریات علمی, 1404; 50(7): 481-491. doi: 10.30476/ijms.2024.103099.3629

Impact of Sappan Wood Extract as Iron Chelator Adjuvant on Iron Concentration and Macrophage Polarization in Rat Spleen

Iranian Journal of Medical Sciences
مقاله 6، دوره 50، شماره 7، مهر 2025، صفحه 481-491    اصل مقاله (2.01 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2024.103099.3629
نویسندگان
Mohammad Ghozali1؛ Ratu Safitri* 2؛ Erick Khristian3؛ Amethyst Puspita Ainni2؛ Yasmi Purnamasari Kuntana2؛ Muhamad Hasan Bashari1؛ Jeri Nobia Purnama3
1Department of Biomedical Sciences, Faculty of Medicine, Padjadjaran University, Jatinangor, West Java 45363, Indonesia
2Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Jatinangor, West Java 45363, Indonesia
3Department of Biotechnology, Faculty of Postgraduate, Padjadjaran University, Bandung, West Java 45363, Indonesia
چکیده
Background: Iron accumulation in the spleen of thalassemia patients disrupts macrophage polarization, impairs immune function, and increases mortality. Sappan wood (Biancaea sappan L.) extract exhibits iron-chelating and immunomodulatory properties, making it a potential adjuvant with Deferiprone (DFP). This study aimed to assess the effects of Sappan wood extract (SWE) as a DFP adjuvant in rat models of iron overload. 
Methods: This experimental study was conducted from January to March 2024 at Padjadjaran University, Indonesia. Thirty-five rats were divided into seven groups: normal, iron overload (IO) induced by iron dextran (ID) at a cumulative dose of 60 mg/Kg body weight (BW), positive control receiving DFP, and four groups receiving DFP+SWE at different doses of 50, 100, 150, and 200 mg/Kg BW. Iron levels and macrophage polarization (pro-inflammatory M1 and anti-inflammatory M2) in the spleen were assessed. 
Results: SWE at 50 mg/Kg BW significantly reduced spleen iron levels to 60.77 ppm/mg and increased M2 intensity (P<0.001 compared to the IO group). At 100 mg/Kg BW, SWE effectively decreased M1 macrophage intensity (P=0.001 compared to the IO group).
Conclusion: SWE at 50 mg/Kg BW was comparable to DFP in reducing the spleen iron levels and M1 macrophage intensity, while 100 mg/Kg BW enhanced M2 macrophage polarization. These findings highlighted SWE’s potential as a therapeutic adjuvant in IO conditions. 

تازه های تحقیق

Mohammad Ghozali (Google Scholar)

Ratu Safitri (Google Scholar)

کلیدواژه‌ها
Deferiprone؛ Macrophage؛ Iron overload؛ Iron chelating agents
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