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A Cross-Sectional Study Comparing Mir-503-5p Expression in Gastric Cancer Patients Before and After Chemotherapy | ||
Iranian Journal of Colorectal Research | ||
مقاله 4، دوره 12، شماره 4، اسفند 2024 اصل مقاله (680.49 K) | ||
نوع مقاله: Research/Original Article | ||
شناسه دیجیتال (DOI): 10.30476/acrr.2025.105786.1241 | ||
نویسندگان | ||
Seyedeh Azra Shamsdin1؛ Sayed Ali Hijazi Hosseini2؛ Hassan Akrami* 1؛ Erfani Nasrollah3؛ Yousef Nikmanesh1؛ Mohammad Reza Fattahi1؛ Mastaneh Zeraatiannejad1 | ||
1Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
2Student research committee, Shiraz university of Medical sciences, Shiraz, Iran. | ||
3Department of Immunology School of Medicine Shiraz university of Medical sciences, Shiraz, Iran. Cancer Immunology & Immunotherapy Group, Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
چکیده | ||
Background: Gastric cancer is the third most fatal cancer among all cancer types. Early diagnosis of this cancer dramatically increases the five-year survival rate of patients. MicroRNAs (miRNAs) play a significant role in tumorigenesis, angiogenesis, invasion, metastasis, and drug resistance in gastric cancer, similar to other cancers. The expression levels of miRNAs can influence tumor progression or suppression. This study aimed to investigate the expression levels of miR-503-5p in the serum of gastric cancer patients both before and after chemotherapy. Methods: This observational study was conducted from 2022 to 2024 at Namazi and Shahid Faghihi Hospitals. Thirty patients with gastric cancer with a definitive diagnosis by gastroenterologists and pathologists and had not received any prior treatment, were included in the study. A blood sample of 5 ml was drawn from each patient and sent to the laboratory for analysis. After the completion of thechemotherapy, blood samples were collected again. The expression level of miR-503-5p was measured using real-time PCR. Data analysis was performed using SPSS version 24, and differences in miR-503-5p expression and clinicopathological characteristics were assessed using ANCOVA and paired ttests. Results: Comparing serum levels of miR-503-5p in gastric cancer patients before and after chemotherapy revealed a 2.12-fold increase in miR-503-5p expression post-treatment (P=0.001). Furthermore, expression levels of miR-503-5p in the serum of patients with metastasis were significantly lower than patients without metastasis (P<0.05). Conclusion: Considering the increased expression of miR-503-5p following treatment in gastric cancer patients, these findings suggest a suppressive role for miR-503-5p in gastric cancer. miR-503-5p may potentially serve as a biomarker for evaluating gastric cancer treatment. | ||
کلیدواژهها | ||
Gastric cancer؛ miR-503-5p؛ chemotherapy؛ gene expression | ||
مراجع | ||
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