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Evaluation of PD-1 Gene Expression Profile and Methylation of the Regulatory Regions in Patients with Ankylosing Spondylitis | ||
| Iranian Journal of Immunology | ||
| دوره 21، شماره 2، شهریور 2024، صفحه 166-175 اصل مقاله (2.03 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2024.101565.2757 | ||
| نویسندگان | ||
| Narjes Soleimanifar1؛ Sara Assaadiasl1؛ Mohammed Sameer Al-Shammari1، 2؛ Abdol-Rahman Rostamian3؛ Maryam Sadr1؛ Sepideh Shahkarami4؛ Hanieh Mojtahedi1؛ Mohammad Hossein Nicknam* 1، 5 | ||
| 1Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 2Hammurabi College of Medicine, University of Babylon, Hillah-Babel, Iraq. | ||
| 3Rheumatology Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 4Research Center for Immunodeficiencies, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 5Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. | ||
| چکیده | ||
| Background: Ankylosing spondylitis (AS) is a chronic autoimmune disorder characterized by the fusion of vertebral joints and axial arthritis. The programmed death-1 (PD-1) inhibitory receptor has a pivotal role in controlling T cell function and may have a significant impact on the pathogenesis of autoimmune diseases such as AS pathogenesis. Objective: To investigate PD-1 gene expression and its epigenetic regulation by detecting methylated CpG islands in the regulatory sites of the gene. This will provide insight into the mechanisms involved in the disease. Methods: 30 AS patients and 30 healthy individuals were examined to detect the 16 CpG islands in intron 1 using bisulfite conversion and methylation-specific PCR technique. In addition, RNA samples were isolated from fresh peripheral blood mononuclear cells (PBMCs), and after complementary DNA (cDNA) synthesis, the expression level of the PD-1 gene was evaluated using Real-Time PCR. Results: The CpG islands located in the intronic zone of the PD-1 gene were hyper-methylated in both the patients with AS and the healthy controls. The gene expression of PD-1 was significantly downregulated in AS patients compared with the controls (p=0.017). A negative correlation between the Bath Ankylosing Spondylitis Disease Activity Index and PD-1 gene expression was also revealed. Conclusion: The low level of PD-1 gene expression is implicated in the pathogenesis of AS. However, in both groups, the methylation level of the intron 1 CpG islands of the PD-1 gene suggests that other regulatory mechanisms are more relevant to PD-1 gene expression than methylation in the intron. | ||
| کلیدواژه‌ها | ||
| Ankylosing Spondylitis؛ DNA Methylation؛ Epigenetics؛ Gene Expression؛ Programmed Cell Death 1 | ||
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آمار تعداد مشاهده مقاله: 646 تعداد دریافت فایل اصل مقاله: 530 |
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