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Akafzadeh, Negar, Baghaei, Fereshteh, Derakhshan, Samira, Moradzadeh Khiavi, Monir, Kharazifard, Mohamadjavad, Aminishakib, Pouyan. (1401). Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia. سامانه مدیریت نشریات علمی, 23(Supplement-September-2022), 377-382. doi: 10.30476/dentjods.2021.91032.1546
Negar Akafzadeh; Fereshteh Baghaei; Samira Derakhshan; Monir Moradzadeh Khiavi; Mohamadjavad Kharazifard; Pouyan Aminishakib. "Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia". سامانه مدیریت نشریات علمی, 23, Supplement-September-2022, 1401, 377-382. doi: 10.30476/dentjods.2021.91032.1546
Akafzadeh, Negar, Baghaei, Fereshteh, Derakhshan, Samira, Moradzadeh Khiavi, Monir, Kharazifard, Mohamadjavad, Aminishakib, Pouyan. (1401). 'Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia', سامانه مدیریت نشریات علمی, 23(Supplement-September-2022), pp. 377-382. doi: 10.30476/dentjods.2021.91032.1546
Akafzadeh, Negar, Baghaei, Fereshteh, Derakhshan, Samira, Moradzadeh Khiavi, Monir, Kharazifard, Mohamadjavad, Aminishakib, Pouyan. Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia. سامانه مدیریت نشریات علمی, 1401; 23(Supplement-September-2022): 377-382. doi: 10.30476/dentjods.2021.91032.1546

Combination of Binary System with Ki67 Immunohistochemical Staining: a Reliable Grading Method for Oral Epithelial Dysplasia

Journal of Dentistry
مقاله 5، دوره 23، Supplement-September-2022، آذر 2022، صفحه 377-382    اصل مقاله (671.2 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/dentjods.2021.91032.1546
نویسندگان
Negar Akafzadeh1؛ Fereshteh Baghaei2؛ Samira Derakhshan3؛ Monir Moradzadeh Khiavi3؛ Mohamadjavad Kharazifard4؛ Pouyan Aminishakib* 3
1Postgraduate Student of Pediatric Dentistry, Department of Pediatric Dentistry, School of Dentistry, Qazvin University of Medical Sciences, Qazvin , Iran
2Retired Professor, Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
3Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
4Researcher, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
چکیده
Statement of the Problem: Oral epithelial dysplasia (OED) is a common potentially malignant lesion of oral cavity that should be managed to prevent likely malignant transformation.
Purpose: Here, we present a combination of binary grading system with complementary immunohistochemical (IHC) staining for Ki67 biomarker to provide a reproducible OED grading system.
Materials and Method: In this cross-sectional study, seventy out of one hundred OED specimens, which were accompanied by IHC stained microscopic slides for Ki67 antigen were evaluated by four independent oral pathologists. Both three-tier and binary grading systems based on WHO microscopic criteria were employed , blindly in a four-step method with at least two-week interval between each observation. Intra- and inter-observational reliability was assessed using Kappa statistical analysis.
Results: OED diagnosis based on binary system showed significant intra-observer reliability comparing to three-tier system without biomarker. Moreover, OED diagnosis based on binary system using Ki67 biomarker showed significant inter-observer reliability comparing to diagnosis in three tier system and based on binary system without Ki67 biomarker showed significant inter-observer reliability comparing to diagnosis based on three-tier system without Ki67.
Conclusion: Here, we found that application of IHC staining for Ki67 biomarker in binary system might provide a more reliable grading method for oral pathologist form different educational background.
کلیدواژه‌ها
Ki-67 Antigen؛ Precancerous Conditions؛ Carcinoma in Situ
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