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Soleimanjahi, Hoorieh, Razavinikoo, Hadi, Fotouhi, Fatemeh, Ardebili, Abdollah. (1396). Antitumor Response to a Codon-Optimized HPV-16 E7/HSP70 Fusion Antigen DNA Vaccine. سامانه مدیریت نشریات علمی, 14(3), 180-191. doi: 10.22034/iji.2017.39308
Hoorieh Soleimanjahi; Hadi Razavinikoo; Fatemeh Fotouhi; Abdollah Ardebili. "Antitumor Response to a Codon-Optimized HPV-16 E7/HSP70 Fusion Antigen DNA Vaccine". سامانه مدیریت نشریات علمی, 14, 3, 1396, 180-191. doi: 10.22034/iji.2017.39308
Soleimanjahi, Hoorieh, Razavinikoo, Hadi, Fotouhi, Fatemeh, Ardebili, Abdollah. (1396). 'Antitumor Response to a Codon-Optimized HPV-16 E7/HSP70 Fusion Antigen DNA Vaccine', سامانه مدیریت نشریات علمی, 14(3), pp. 180-191. doi: 10.22034/iji.2017.39308
Soleimanjahi, Hoorieh, Razavinikoo, Hadi, Fotouhi, Fatemeh, Ardebili, Abdollah. Antitumor Response to a Codon-Optimized HPV-16 E7/HSP70 Fusion Antigen DNA Vaccine. سامانه مدیریت نشریات علمی, 1396; 14(3): 180-191. doi: 10.22034/iji.2017.39308

Antitumor Response to a Codon-Optimized HPV-16 E7/HSP70 Fusion Antigen DNA Vaccine

Iranian Journal of Immunology
مقاله 1، دوره 14، شماره 3، آذر 2017، صفحه 180-191    اصل مقاله (364.29 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2017.39308
نویسندگان
Hoorieh Soleimanjahi1؛ Hadi Razavinikoo2؛ Fatemeh Fotouhi3؛ Abdollah Ardebili2
1Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran
چکیده
Background: Vaccines based on virus-like particles are effective against Human Papilloma Virus (HPV) infection; however, they have not shown a therapeutic effect against HPV-associated diseases. New immunotherapy strategies based on immune responses against tumor antigens can positively affect the clearance of HPV-associated lesions. Objective: To generate two therapeutic fusion DNA vaccines (optimizedE7/mouseHSP70 and wildE7/mouseHSP70) to induce antitumor specific responses in mice models. Methods: Mice were immunized with recombinant DNA vaccines. The splenocytes of immunized mice were collected and lactate dehydrogenase and IFN-γ productions were measured after three injections in order to evaluate cytotoxic T lymphocytes (CTLs) activity. MTT assay was carried out for lymphocyte stimulation. Results: The fusion DNA vaccines, specifically uE7-HSP70, elicited varying levels of IFN-γ and CTLs responses compared to the control group (P<0.05). Furthermore, antitumor response and tumor size reduction in fusion DNA vaccines groups were significantly higher than in the negative control group (P<0.05). Conclusion: It is concluded that our fusion DNA vaccines considerably enhanced specific cellular responses against HPV tumor model. In addition, optimized E7 showed a notable immunogenicity and inhibitory effect on the reduction of tumor size.
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