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El Husseiny, Noha Mohamed, Attya Elkak, Walaa Abd El Fattah, El Ansary, Mervat Said, El Khateeb, Engy Mohamed Abd El Aziz, Wagih Mattar, Mervat, El Demerdash, Doaa Mohamed. (1395). The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia. سامانه مدیریت نشریات علمی, 14(1), 1-12. doi: 10.22034/iji.2017.39284
Noha Mohamed El Husseiny; Walaa Abd El Fattah Attya Elkak; Mervat Said El Ansary; Engy Mohamed Abd El Aziz El Khateeb; Mervat Wagih Mattar; Doaa Mohamed El Demerdash. "The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia". سامانه مدیریت نشریات علمی, 14, 1, 1395, 1-12. doi: 10.22034/iji.2017.39284
El Husseiny, Noha Mohamed, Attya Elkak, Walaa Abd El Fattah, El Ansary, Mervat Said, El Khateeb, Engy Mohamed Abd El Aziz, Wagih Mattar, Mervat, El Demerdash, Doaa Mohamed. (1395). 'The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia', سامانه مدیریت نشریات علمی, 14(1), pp. 1-12. doi: 10.22034/iji.2017.39284
El Husseiny, Noha Mohamed, Attya Elkak, Walaa Abd El Fattah, El Ansary, Mervat Said, El Khateeb, Engy Mohamed Abd El Aziz, Wagih Mattar, Mervat, El Demerdash, Doaa Mohamed. The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia. سامانه مدیریت نشریات علمی, 1395; 14(1): 1-12. doi: 10.22034/iji.2017.39284

The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia

Iranian Journal of Immunology
مقاله 1، دوره 14، شماره 1، خرداد 2017، صفحه 1-12    اصل مقاله (529.66 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2017.39284
نویسندگان
Noha Mohamed El Husseiny1؛ Walaa Abd El Fattah Attya Elkak2؛ Mervat Said El Ansary2؛ Engy Mohamed Abd El Aziz El Khateeb2؛ Mervat Wagih Mattar1؛ Doaa Mohamed El Demerdash3
1Clinical Haematology Unit, Internal Medicine, Cairo University, Cairo, Egypt
2Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
3Clinical Haematology Unit, Internal Medicine, Cairo University, Cairo, Egypt
چکیده
Background: Generation of monocyte-derived dendritic cells (MDDC) is induced in the presence of GM-CSF and IL-4, and a maturation stimulus is added to the monocyte culture to obtain mature Dendritic Cells (DCs) suitable for therapy. TNF-α is the most common cytokine used for activating DCs and generating mature MDDC either alone or in combination with other cytokines. Objective: To compare effects of traditional cytokine cocktail (TNF-α + IL-1β) versus TLR4-agonist monophosphoryl lipid A on the viability, phenotype, cytokine profile and functionality of MDDC. Methods: The study included 32 individuals; twenty Acute Myeloid Leukaemia (AML) cases in complete remission and 12 healthy volunteers. They were divided into 3 groups: Group 1: control group: 12 subjects to measure the baseline levels of all markers in the monocytic preparation; Group 2: cytokine cocktail (TNF-α) group, which included 10 AML subjects; Group 3: MPLA group which included 10 AML subjects. Results: TNF-α group showed higher expression of CD83 than MPLA group indicating higher capacity to induce DC maturation but both were similar in CD86, CCR7 and IL-10 expression. Preparation of dendritic cells from AML cases in remission and loading them with tumor peptides was successful. Conclusion: The effect of MPLA in DC maturation is comparable with traditional DC maturation cocktail.
کلیدواژه‌ها
DCs Maturation؛ Monocyte-Derived DCs
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