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Sharifzadeh, Sedigheh, Modjtahedi, Helmout, Jedi Tehrani, Mahmood, Ghaderi, Abbas. (1386). Production and Characterization of a Monoclonal Antibody against an Antigen on the Surface of Non-Small Cell Carcinoma of the Lung. سامانه مدیریت نشریات علمی, 4(4), 206-214. doi: 10.22034/iji.2007.17199
Sedigheh Sharifzadeh; Helmout Modjtahedi; Mahmood Jedi Tehrani; Abbas Ghaderi. "Production and Characterization of a Monoclonal Antibody against an Antigen on the Surface of Non-Small Cell Carcinoma of the Lung". سامانه مدیریت نشریات علمی, 4, 4, 1386, 206-214. doi: 10.22034/iji.2007.17199
Sharifzadeh, Sedigheh, Modjtahedi, Helmout, Jedi Tehrani, Mahmood, Ghaderi, Abbas. (1386). 'Production and Characterization of a Monoclonal Antibody against an Antigen on the Surface of Non-Small Cell Carcinoma of the Lung', سامانه مدیریت نشریات علمی, 4(4), pp. 206-214. doi: 10.22034/iji.2007.17199
Sharifzadeh, Sedigheh, Modjtahedi, Helmout, Jedi Tehrani, Mahmood, Ghaderi, Abbas. Production and Characterization of a Monoclonal Antibody against an Antigen on the Surface of Non-Small Cell Carcinoma of the Lung. سامانه مدیریت نشریات علمی, 1386; 4(4): 206-214. doi: 10.22034/iji.2007.17199

Production and Characterization of a Monoclonal Antibody against an Antigen on the Surface of Non-Small Cell Carcinoma of the Lung

Iranian Journal of Immunology
مقاله 3، دوره 4، شماره 4، اسفند 2007، صفحه 206-214    اصل مقاله (466.35 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2007.17199
نویسندگان
Sedigheh Sharifzadeh1، 2؛ Helmout Modjtahedi3؛ Mahmood Jedi Tehrani4؛ Abbas Ghaderi2، 5
1Department of Laboratory Science, Paramedical School
2Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
3Postgraduate Medical School, University of Surrey, Guildford, United–Kingdom
4Avecina Institute, Tehran, Iran
5Shiraz Institute for Cancer Research, Shiraz, Iran
چکیده
Background: Lung carcinoma is a multiple type cancer comprising of small cell and non-small cell carcinomas (NSCLC). For therapeutic and diagnostic purposes, serum monoclonal antibodies have been produced against lung cancer.
Objective: To charac-terize a murine monoclonal antibody (ME3D11) reactive with human NSCLC.
Methods: A murine monoclonal antibody (ME3D11) reactive with human NSCLC was selected after immunization of BALB/c mice with a human large cell carcinoma with neuroen-docrine differentiation, and was tested by immunofloursence staining and Western blot analysis.
Results: Our study showed that the antigen recognized by ME3D11 antibody was a cell surface antigen of 170kDa. This antigen is expressed on the cell surface of all NSCLC and a few carcinoma cell lines. In contrast, this antigen is neither expressed on the cell surface of human sarcoma, nor on the hematopoietic and normal cell lines. This anti-body had no effect on spontaneous proliferation of Mehr-80 cell line in vitro.
Conclusion: High degree of binding of this monoclonal antibody to NSCLC and some other carci-noma cells warrants further studies on its potential use in diagnosis and therapy of can-cer by conjugation to drugs, toxins or radionuclides.
کلیدواژه‌ها
NSCLC؛ ME3D11؛ Mehr-80
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