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Mansour, Durria, M. Abass, Elfadil, Mahamoud, Abdelhafeiz, Harith, Abdallah el. (1388). Qualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan. سامانه مدیریت نشریات علمی, 6(4), 208-215. doi: 10.22034/iji.2009.17094
Durria Mansour; Elfadil M. Abass; Abdelhafeiz Mahamoud; Abdallah el Harith. "Qualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan". سامانه مدیریت نشریات علمی, 6, 4, 1388, 208-215. doi: 10.22034/iji.2009.17094
Mansour, Durria, M. Abass, Elfadil, Mahamoud, Abdelhafeiz, Harith, Abdallah el. (1388). 'Qualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan', سامانه مدیریت نشریات علمی, 6(4), pp. 208-215. doi: 10.22034/iji.2009.17094
Mansour, Durria, M. Abass, Elfadil, Mahamoud, Abdelhafeiz, Harith, Abdallah el. Qualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan. سامانه مدیریت نشریات علمی, 1388; 6(4): 208-215. doi: 10.22034/iji.2009.17094

Qualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan

Iranian Journal of Immunology
مقاله 6، دوره 6، شماره 4، اسفند 2009، صفحه 208-215    اصل مقاله (163.72 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2009.17094
نویسندگان
Durria Mansour1؛ Elfadil M. Abass1؛ Abdelhafeiz Mahamoud1؛ Abdallah el Harith* 2
1Laboratory of Biomedical Research, Ahfad University for Women, P.O. Box 167, Omdurman, Sudan
2Retired Microbiologist, Wijngaard 155, 8212 CJ, Lelystad, The Netherlands
چکیده
Background: Until now, the comparison of the rK39 strip test (RKT) and direct ag-glutination test (DAT) for detection of visceral leishmaniasis (VL) is exclusively based on either positive or negative qualification of the reaction outcome.
Objective: In this study, we compared the diagnostic performance of RKT and DAT for VL both qualitatively and semi-quantitatively.
Methods: For comparison based on semi-quantitative grounds, the execution of RKT and DAT was according to the standard procedures. For comparison on semi-qualitative grounds with DAT, the RKT was ap-plied to aliquots from positive samples that were two-fold serially diluted in saline to determine, as for the DAT, the end-point reaction in RKT.
Results: While qualita-tively both RKT and DAT demonstrated comparable reliability for VL detection (sen-sitivity = 96% and specificity = 98.7% or 99.3%), no significant correlation (r = 0.13) could be established between intensities of their positive reactions in 25 cases studied. A negative correlation was further determined in those 25 VL cases between the posi-tive intensities of the RKT and antibody levels measured semi-quantitatively with the same procedure (r = -0.36) or the DAT (r = -0.30). Irrespective of the low, moderate or high antibody levels measured with RKT (<1:8 and 1:16-1:32 >1:256) or DAT (< 1:25,600 and 1:51,200- 1:409,600 > 1:3,276,800) in patients with confirmed or uncon-firmed VL infection, exclusively strong positive intensities were obtained with RKT.
Conclusion: For further optimizing diagnosis and simultaneously assessing magni-tude of immune response to L. donovani infection in Sudanese patients, the combined application of RKT and DAT is recommended.
کلیدواژه‌ها
visceral leishmaniasis؛ rK39؛ Agglutination Test
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