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Ghatei, Mohammad Amin, Hatam, Gholamreza, Hosseini, Seyed Mohammad Hossein, Sarkari, Bahador. (1388). Performance of Latex Agglutination Test (KAtex) In Diagnosis of Visceral Leishmaniasis in Iran. سامانه مدیریت نشریات علمی, 6(4), 202-207. doi: 10.22034/iji.2009.17093
Mohammad Amin Ghatei; Gholamreza Hatam; Seyed Mohammad Hossein Hosseini; Bahador Sarkari. "Performance of Latex Agglutination Test (KAtex) In Diagnosis of Visceral Leishmaniasis in Iran". سامانه مدیریت نشریات علمی, 6, 4, 1388, 202-207. doi: 10.22034/iji.2009.17093
Ghatei, Mohammad Amin, Hatam, Gholamreza, Hosseini, Seyed Mohammad Hossein, Sarkari, Bahador. (1388). 'Performance of Latex Agglutination Test (KAtex) In Diagnosis of Visceral Leishmaniasis in Iran', سامانه مدیریت نشریات علمی, 6(4), pp. 202-207. doi: 10.22034/iji.2009.17093
Ghatei, Mohammad Amin, Hatam, Gholamreza, Hosseini, Seyed Mohammad Hossein, Sarkari, Bahador. Performance of Latex Agglutination Test (KAtex) In Diagnosis of Visceral Leishmaniasis in Iran. سامانه مدیریت نشریات علمی, 1388; 6(4): 202-207. doi: 10.22034/iji.2009.17093

Performance of Latex Agglutination Test (KAtex) In Diagnosis of Visceral Leishmaniasis in Iran

Iranian Journal of Immunology
مقاله 5، دوره 6، شماره 4، اسفند 2009، صفحه 202-207    اصل مقاله (132.81 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2009.17093
نویسندگان
Mohammad Amin Ghatei1؛ Gholamreza Hatam1؛ Seyed Mohammad Hossein Hosseini2؛ Bahador Sarkari* 1
1Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Razi Vaccine and Serum Research Institute, South Branch, Shiraz, Iran
چکیده
Background: Visceral leishmaniasis (VL) is an endemic disease in some parts of Iran. Many techniques have been used for diagnosis of VL, among which the urine based la-tex agglutination test (KAtex) is a promising one.
Objective: To compare three diag-nostic tests of VL including KAtex, ELISA and Direct Agglutination Test (DAT) in VL patients and healthy controls in the south west of Iran.
Methods: Serum (n = 29) and urine samples (n = 31) were collected from parasitologically confirmed VL patients. Control samples were obtained from healthy individuals (n = 61) and also from patients with infectious diseases other than VL. The collected serum samples were tested by DAT and ELISA using crude antigen from promastigotes of Leishmania infantum and the urine samples were tested by KAtex.
Results: Sensitivity and specificity of KAtex for diagnosis of VL was found to be 83.9% and 100%, respectively. Sensitivities of DAT and ELISA were 93.1% and 86.2% and their specificities were 100% and 90.5%, respectively.
Conclusion: KAtex yielded a satisfactory sensitivity and specificity in di-agnosis of VL in Iran and can be recommended as a rapid, field applicable and reliable test for diagnosis of VL in this region.
کلیدواژه‌ها
visceral leishmaniasis؛ ELISA؛ Agglutination Test
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