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Ghashghaie Mansour, Andisheh, Ghaffari, Seyyed Hamidollah, Ali-moghadam, Kamran, Ghavamzadeh, Ardeshir. (1385). Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors. سامانه مدیریت نشریات علمی, 3(2), 95-98. doi: 10.22034/iji.2006.16974
Andisheh Ghashghaie Mansour; Seyyed Hamidollah Ghaffari; Kamran Ali-moghadam; Ardeshir Ghavamzadeh. "Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors". سامانه مدیریت نشریات علمی, 3, 2, 1385, 95-98. doi: 10.22034/iji.2006.16974
Ghashghaie Mansour, Andisheh, Ghaffari, Seyyed Hamidollah, Ali-moghadam, Kamran, Ghavamzadeh, Ardeshir. (1385). 'Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors', سامانه مدیریت نشریات علمی, 3(2), pp. 95-98. doi: 10.22034/iji.2006.16974
Ghashghaie Mansour, Andisheh, Ghaffari, Seyyed Hamidollah, Ali-moghadam, Kamran, Ghavamzadeh, Ardeshir. Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors. سامانه مدیریت نشریات علمی, 1385; 3(2): 95-98. doi: 10.22034/iji.2006.16974

Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors

Iranian Journal of Immunology
مقاله 8، دوره 3، شماره 2 - شماره پیاپی 9، شهریور 2006، صفحه 95-98    اصل مقاله (48.42 K)
نوع مقاله: Short Communication
شناسه دیجیتال (DOI): 10.22034/iji.2006.16974
نویسندگان
Andisheh Ghashghaie Mansour* ؛ Seyyed Hamidollah Ghaffari؛ Kamran Ali-moghadam؛ Ardeshir Ghavamzadeh
Hematology, Oncology, and Bone Marrow Transplantation Research Center, Tehran University of Medical sciences, Tehran, Iran
چکیده
Background: HLA compatibility between transplant donor and recipient is one of the major determinants of transplant outcome.
Objective: To determine HLA class I by PCR- Sequence-Specific Oligonucleotide Probe (PCR-SSOP) in cord blood donors.
Methods: Genomic DNA of 142 cord blood samples registered at the Cord Blood Bank of Iran at Hematology, Oncology, and Bone Marrow Transplantation Research Center, was prepared and HLA class I was determined by the PCR-SSOP.
Results: A total of 284 HLA-A alleles was identified of which A*02 and A*24 were the most common. Among 284 HLA-B and HLA-C alleles, B*35, B*51, Cw*4 and Cw*12 were the most frequent alleles in the studied population.
Conclusion: Amplification of HLA loci with PCR-SSOP has proved to be a reliable method for HLA-A, -B and -C genotyping.
کلیدواژه‌ها
HLA Typing؛ PCR-SSOP؛ Cord blood؛ HLA Class I
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