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Vahedi, Fatemeh, Taiebi Meibody, Naser, KianiZadeh, Mahdi, Mahmoudi, Mahmoud. (1384). Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine. سامانه مدیریت نشریات علمی, 2(3), 134-140. doi: 10.22034/iji.2005.16904
Fatemeh Vahedi; Naser Taiebi Meibody; Mahdi KianiZadeh; Mahmoud Mahmoudi. "Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine". سامانه مدیریت نشریات علمی, 2, 3, 1384, 134-140. doi: 10.22034/iji.2005.16904
Vahedi, Fatemeh, Taiebi Meibody, Naser, KianiZadeh, Mahdi, Mahmoudi, Mahmoud. (1384). 'Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine', سامانه مدیریت نشریات علمی, 2(3), pp. 134-140. doi: 10.22034/iji.2005.16904
Vahedi, Fatemeh, Taiebi Meibody, Naser, KianiZadeh, Mahdi, Mahmoudi, Mahmoud. Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine. سامانه مدیریت نشریات علمی, 1384; 2(3): 134-140. doi: 10.22034/iji.2005.16904

Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine

Iranian Journal of Immunology
مقاله 2، دوره 2، شماره 3 - شماره پیاپی 6، آذر 2005، صفحه 134-140    اصل مقاله (368.89 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2005.16904
نویسندگان
Fatemeh Vahedi1، 2؛ Naser Taiebi Meibody3؛ Mahdi KianiZadeh2؛ Mahmoud Mahmoudi* 1
1Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2Razi Vaccine and Serum Research Institute, Mashhad, Iran
3Department of Pathology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity.
Objective: To evaluate the use of a DNA immunization strategy for protection against anthrax, a plasmid was constructed.
Methods: The partialsequence of protective antigen of Bacillus anthracis, amino acids 175-764, as a potent immunogenic target was selected. The DNA encoding this segment was utilized in the construction of pcDNA3.1+PA plasmid. After intramuscular injection of rats with pcDNA3.1+PA plasmid, the expression of PA was assessed by RT-PCR and immunohistochemistry at RNA and protein levels, respectively. We also evaluated the presence of anti-PA antibodies in sera of immunized mice with pcDNA3.1+PA construct using immunoblotting.
Results: The integrity of pcDNA3.1+PA construct was confirmed with restriction analysis and sequencing. The expression of PA was detected at RNA and protein levels. The presence of anti-PA antibodies in immunized mice with pcDNA3.1+PA construct was also confirmed.
Conclusion: Our results indicate that pcDNA3.1+PA eukaryotic expressing vector could express PA antigen, induce antibody response and may be used as a candidate for DNA vaccine against anthrax.
کلیدواژه‌ها
Bacillus Anthracis؛ Protective Antigen (PA)؛ DNA vaccine
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