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Meshkat, Zahra, Soleimanjahi, Hoorieh, Mirshahabi, Hessam, Meshkat, Mojtaba, Kheiandish, Maryam, Mohammad Hassan, Zuhair. (1390). Strong Immune Responses Induced by a DNA Vaccine Containing HPV16 Truncated E7 C-terminal Linked to HSP70 Gene. سامانه مدیریت نشریات علمی, 8(2), 65-75. doi: 10.22034/iji.2011.16890
Zahra Meshkat; Hoorieh Soleimanjahi; Hessam Mirshahabi; Mojtaba Meshkat; Maryam Kheiandish; Zuhair Mohammad Hassan. "Strong Immune Responses Induced by a DNA Vaccine Containing HPV16 Truncated E7 C-terminal Linked to HSP70 Gene". سامانه مدیریت نشریات علمی, 8, 2, 1390, 65-75. doi: 10.22034/iji.2011.16890
Meshkat, Zahra, Soleimanjahi, Hoorieh, Mirshahabi, Hessam, Meshkat, Mojtaba, Kheiandish, Maryam, Mohammad Hassan, Zuhair. (1390). 'Strong Immune Responses Induced by a DNA Vaccine Containing HPV16 Truncated E7 C-terminal Linked to HSP70 Gene', سامانه مدیریت نشریات علمی, 8(2), pp. 65-75. doi: 10.22034/iji.2011.16890
Meshkat, Zahra, Soleimanjahi, Hoorieh, Mirshahabi, Hessam, Meshkat, Mojtaba, Kheiandish, Maryam, Mohammad Hassan, Zuhair. Strong Immune Responses Induced by a DNA Vaccine Containing HPV16 Truncated E7 C-terminal Linked to HSP70 Gene. سامانه مدیریت نشریات علمی, 1390; 8(2): 65-75. doi: 10.22034/iji.2011.16890

Strong Immune Responses Induced by a DNA Vaccine Containing HPV16 Truncated E7 C-terminal Linked to HSP70 Gene

Iranian Journal of Immunology
مقاله 1، دوره 8، شماره 2، شهریور 2011، صفحه 65-75    اصل مقاله (302.23 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2011.16890
نویسندگان
Zahra Meshkat1؛ Hoorieh Soleimanjahi* 1؛ Hessam Mirshahabi1؛ Mojtaba Meshkat2؛ Maryam Kheiandish3؛ Zuhair Mohammad Hassan1
1Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran
2School of Medicine, Islamic Azad University, Mashhad
3Blood Transfusion Center, Tehran, Iran
چکیده
Background: Vaccines capable of controlling tumor virus based infections are found difficult to develop due to the consistence latent infection in the host. DNA vaccines are attractive tools for the development of HPV vaccines and inducing antigen-specific immunity owing to the stability, simplicity of delivery, safety and cost effectiveness. However, there is a need to increase their potency by procedures such as using HSP70 gene as an adjuvant.
Objective: To evaluate a DNA vaccine containing HPV16 truncated E7 C-terminal cytotoxic T-lymphocyte epitopes linked to HSP70 gene (HSP70-tE7) in an animal model.
Methods: Mice were immunized with the plasmid DNA after pre-treatment with cardiotoxin. The splenocytes of immunized mice were then tested for CTL activity by detecting the apoptosis and necrosis in target cells, cytokine production by ELISA, CD4 and CD8 frequencies by flow cytometry, and lymphocyte stimulation by MTT assay.
Results: The recombinant expression vector was able to elicit immune responses close to that of full length E7 complete gene. Although the use of a small part of a target antigen can induce immune responses equivalent to the full length antigen, it fails to elicit statistically significant stronger immune responses when fused with HSP70 compared to the complete E7 gene alone.
Conclusion: The potent immunogenicity of HPV16 E7 was preserved in the HSP70-tE7 vaccine and may represent a target of choice for the therapeutic vaccination strategies. However, to improve the immunogenicity polytope DNA vaccines which elicit multiple effector and memory CTL responses should be considered in future studies of DNAbased cancer vaccines.
کلیدواژه‌ها
CTL Epitope؛ DNA vaccine؛ E7؛ HSP70؛ Human papillomavirus type 16
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