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Farjadian, Shirin, Norouzian, Marzie, Younesi, Vahid, Ebrahimpour, Azin, Lotfi, Ramin. (1392). Hyperthermia Increases Natural Killer Cell Cytotoxicity against SW-872 Liposarcoma Cell Line. سامانه مدیریت نشریات علمی, 10(2), 93-102. doi: 10.22034/iji.2013.16816
Shirin Farjadian; Marzie Norouzian; Vahid Younesi; Azin Ebrahimpour; Ramin Lotfi. "Hyperthermia Increases Natural Killer Cell Cytotoxicity against SW-872 Liposarcoma Cell Line". سامانه مدیریت نشریات علمی, 10, 2, 1392, 93-102. doi: 10.22034/iji.2013.16816
Farjadian, Shirin, Norouzian, Marzie, Younesi, Vahid, Ebrahimpour, Azin, Lotfi, Ramin. (1392). 'Hyperthermia Increases Natural Killer Cell Cytotoxicity against SW-872 Liposarcoma Cell Line', سامانه مدیریت نشریات علمی, 10(2), pp. 93-102. doi: 10.22034/iji.2013.16816
Farjadian, Shirin, Norouzian, Marzie, Younesi, Vahid, Ebrahimpour, Azin, Lotfi, Ramin. Hyperthermia Increases Natural Killer Cell Cytotoxicity against SW-872 Liposarcoma Cell Line. سامانه مدیریت نشریات علمی, 1392; 10(2): 93-102. doi: 10.22034/iji.2013.16816

Hyperthermia Increases Natural Killer Cell Cytotoxicity against SW-872 Liposarcoma Cell Line

Iranian Journal of Immunology
مقاله 4، دوره 10، شماره 2، شهریور 2013، صفحه 93-102    اصل مقاله (1.04 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2013.16816
نویسندگان
Shirin Farjadian1؛ Marzie Norouzian1؛ Vahid Younesi1؛ Azin Ebrahimpour1؛ Ramin Lotfi2، 3
1Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
2Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service BadenWürttemberg-Hessen
3Institute of Transfusion Medicine, University of Ulm, Ulm, Germany
چکیده
Background: Although there is convincing data in support of the effectiveness of hyperthermia in tumor therapy, the molecular mechanisms underlying the clinical effects of hyperthermia are still poorly understood.
Objective: To investigate natural killer (NK) cell cytotoxicity against heat-treated SW-872 and HeLa tumor cell lines.
Methods: NKG2D ligands and HLA class I transcription were examined using quantitative real-time PCR in treated tumor cell lines at 0, 2, 4, 6 and 12 h following thermal treatment at 39C and 42C for 1 h. The expression of MICA/B, ULBP1 and ULBP2 were also determined by flow cytometry. NK92-MI cytotoxic activity against heat-treated target cell lines was assessed by LDH release as well as annexin-V and 7-AAD assays.
Results: Our results showed that heat treatment at 39C improved the cytolytic activity of NK cells against SW-872 cells without increasing NKG2D ligand concentration or decreasing HLA class I levels.
Conclusion: The observed increase in the cytotoxicity of NK cells against SW-872 cells after hyperthermia does not coincide with changes in MICA/B, ULBP1 and ULBP2 ligands of NKG2, however, the expression of other ligands in target cells may have made the cells susceptible to the cytotoxic effect of NK cells.
کلیدواژه‌ها
Cytotoxicity؛ Hyperthermia؛ Liposarcoma؛ NKG2D Ligands؛ SW-872
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