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Basiri, Ezzatallah, Mohseni Ardehali, Sadreddin, Doroudchi, Mehrnoosh, Mahboodi, Fereidoun, Kharazmi, Arsalan, Ghaderi, Abbas. (1383). Production and Characterization of Murine Monoclonal Antibodies to Leishmania Gp63 Antigen. سامانه مدیریت نشریات علمی, 1(3), 194-199. doi: 10.22034/iji.2004.16815
Ezzatallah Basiri; Sadreddin Mohseni Ardehali; Mehrnoosh Doroudchi; Fereidoun Mahboodi; Arsalan Kharazmi; Abbas Ghaderi. "Production and Characterization of Murine Monoclonal Antibodies to Leishmania Gp63 Antigen". سامانه مدیریت نشریات علمی, 1, 3, 1383, 194-199. doi: 10.22034/iji.2004.16815
Basiri, Ezzatallah, Mohseni Ardehali, Sadreddin, Doroudchi, Mehrnoosh, Mahboodi, Fereidoun, Kharazmi, Arsalan, Ghaderi, Abbas. (1383). 'Production and Characterization of Murine Monoclonal Antibodies to Leishmania Gp63 Antigen', سامانه مدیریت نشریات علمی, 1(3), pp. 194-199. doi: 10.22034/iji.2004.16815
Basiri, Ezzatallah, Mohseni Ardehali, Sadreddin, Doroudchi, Mehrnoosh, Mahboodi, Fereidoun, Kharazmi, Arsalan, Ghaderi, Abbas. Production and Characterization of Murine Monoclonal Antibodies to Leishmania Gp63 Antigen. سامانه مدیریت نشریات علمی, 1383; 1(3): 194-199. doi: 10.22034/iji.2004.16815

Production and Characterization of Murine Monoclonal Antibodies to Leishmania Gp63 Antigen

Iranian Journal of Immunology
مقاله 8، دوره 1، شماره 3، اسفند 2004، صفحه 194-199    اصل مقاله (204.74 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2004.16815
نویسندگان
Ezzatallah Basiri* 1؛ Sadreddin Mohseni Ardehali2؛ Mehrnoosh Doroudchi3؛ Fereidoun Mahboodi4؛ Arsalan Kharazmi5؛ Abbas Ghaderi3
1Bcteriology and Virology
2Parasitology and Mycology
3Immunology , School of Medicine, Shiraz University of Medical Sciences ,Shiraz, Iran
4Pasteur Institute of Iran
5Staten Serum Institut Klinisk Microbiologisk, Afdeling Righospitalet AFB7806 Copenhagen, Denmark
چکیده
Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated recombinant gp63 molecules were studied by an ELISA assay. These two antibodies reacted with the crude soluble antigens prepared from 4 reference strains of Leishmania, 10 isolates from the patients, purified gp63 and recombinant gp63 molecules. However, no reaction with several non-leishmanial antigens was observed. Reaction of both antibodies with the intact recombinant gp63 and truncated molecules were compared.
Results: The results indicated that the two antibodies specifically recognize two different epitopes on the gp63 molecule.
Conclusion: Possible applications of such antibodies in searching for immunogenic epitopes are discussed.
کلیدواژه‌ها
gp63؛ Leishmania؛ Monoclonal antibody
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