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Mohammadi, Mohsen, Kianmehr, Zahra, Kaboudanian Ardestani, Sussan, Gharegozlou, Behnaz. (1393). Improved Immunogenicity of Tetanus Toxoid by Brucella abortus S19 LPS Adjuvant. سامانه مدیریت نشریات علمی, 11(3), 189-199. doi: 10.22034/iji.2014.16779
Mohsen Mohammadi; Zahra Kianmehr; Sussan Kaboudanian Ardestani; Behnaz Gharegozlou. "Improved Immunogenicity of Tetanus Toxoid by Brucella abortus S19 LPS Adjuvant". سامانه مدیریت نشریات علمی, 11, 3, 1393, 189-199. doi: 10.22034/iji.2014.16779
Mohammadi, Mohsen, Kianmehr, Zahra, Kaboudanian Ardestani, Sussan, Gharegozlou, Behnaz. (1393). 'Improved Immunogenicity of Tetanus Toxoid by Brucella abortus S19 LPS Adjuvant', سامانه مدیریت نشریات علمی, 11(3), pp. 189-199. doi: 10.22034/iji.2014.16779
Mohammadi, Mohsen, Kianmehr, Zahra, Kaboudanian Ardestani, Sussan, Gharegozlou, Behnaz. Improved Immunogenicity of Tetanus Toxoid by Brucella abortus S19 LPS Adjuvant. سامانه مدیریت نشریات علمی, 1393; 11(3): 189-199. doi: 10.22034/iji.2014.16779

Improved Immunogenicity of Tetanus Toxoid by Brucella abortus S19 LPS Adjuvant

Iranian Journal of Immunology
مقاله 5، دوره 11، شماره 3، آذر 2014، صفحه 189-199    اصل مقاله (313.21 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2014.16779
نویسندگان
Mohsen Mohammadi1؛ Zahra Kianmehr1؛ Sussan Kaboudanian Ardestani* 1؛ Behnaz Gharegozlou2
1Immunology Lab, Institute of Biochemistry and Biophysics, University of Tehran
2Faculty of Allied Health, Department of Immunology, Tehran University of Medical Science, Tehran, Iran
چکیده
Background: Adjuvants are used to increase the immunogenicity of new generation vaccines, especially those based on recombinant proteins. Despite immunostimulatory properties, the use of bacterial lipopolysaccharide (LPS) as an adjuvant has been hampered due to its toxicity and pyrogenicity. Brucella abortus LPS is less toxic and has no pyrogenic properties compared to LPS from other gram negative bacteria.
Objectives: To evaluate the adjuvant effect of B. abortus (vaccine strain, S19) LPS for tetanus toxoid antigen (TT) and to investigate the protective effect of different tetanus vaccine preparations.
Methods: LPS was extracted and purified from B. abortus S19 and KDO, glycan, phosphate content, and protein contamination were measured. Adipic acid dihydrazide (ADH) was used as a linker for the conjugation of TT to LPS. Different amounts of B. abortus LPS, TT, TT conjugated with LPS, and TT mixed with LPS or complete Freund’s adjuvant (CFA) were injected into mice and antibody production against TT was measured. The protective effect of induced antibodies was determined by LD50.
Results: Immunization of mice with TT+LPS produced the highest anti-TT antibody titer in comparison to the group immunized with TT without any adjuvant or the groups immunized with TT-LPS or TT+CFA. Tetanus toxid-S19 LPS also produced a 100% protective effect against TT in immunized mice.
Conclusion: These data indicate that B. abortus LPS enhances the immune responses to TT and suggest the possible use of B. abortus LPS as an adjuvant in vaccine preparations.
کلیدواژه‌ها
Adjuvant؛ Brucella abortus S19؛ LPS؛ Tetanus Toxoid Antigen
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