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Li, Yang, Zhang, Lu, Wang, Shouyu, Shi, Peng, Qu, Wei. (1393). Optimizing Dendritic Cell Preparation for Fusion with Melanoma Cells. سامانه مدیریت نشریات علمی, 11(3), 166-176. doi: 10.22034/iji.2014.16777
Yang Li; Lu Zhang; Shouyu Wang; Peng Shi; Wei Qu. "Optimizing Dendritic Cell Preparation for Fusion with Melanoma Cells". سامانه مدیریت نشریات علمی, 11, 3, 1393, 166-176. doi: 10.22034/iji.2014.16777
Li, Yang, Zhang, Lu, Wang, Shouyu, Shi, Peng, Qu, Wei. (1393). 'Optimizing Dendritic Cell Preparation for Fusion with Melanoma Cells', سامانه مدیریت نشریات علمی, 11(3), pp. 166-176. doi: 10.22034/iji.2014.16777
Li, Yang, Zhang, Lu, Wang, Shouyu, Shi, Peng, Qu, Wei. Optimizing Dendritic Cell Preparation for Fusion with Melanoma Cells. سامانه مدیریت نشریات علمی, 1393; 11(3): 166-176. doi: 10.22034/iji.2014.16777

Optimizing Dendritic Cell Preparation for Fusion with Melanoma Cells

Iranian Journal of Immunology
مقاله 3، دوره 11، شماره 3، آذر 2014، صفحه 166-176    اصل مقاله (1.14 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2014.16777
نویسندگان
Yang Li1؛ Lu Zhang2؛ Shouyu Wang1؛ Peng Shi1؛ Wei Qu* 1
1Department of Orthopaedics
2Department of Maternity, First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, China
چکیده
Background: Fusion of dendritic cells (DCs) with melanoma cells could reinforce the antigenicity of tumors as a strategy for the treatment of malignant melanoma. However, the insufficient quantity of DCs and the low fusion efficiency limits the development of such approach.
Objective: To define the dosage of the stimulating factors as well as the induction condition for the optimal DCs preparation and cell fusion. Methods: DCs were generated from murine bone marrow cells, and cultured with four different concentrations of the granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). DCs were confirmed to be mature by detecting the expression of MHC-II, CD11c, CD80, and CD83 by flowcytometry. DCs-melanoma fusion cells were generated using polyethylene glycols (PEG) with different molecular weights and the fusion efficiency was detected by fluorescence-activated cell sorter (FACS).
Results: The largest quantity of DCs was found when cells were cultured with 1000 U/ ml of GM-CSF and 500 U/ml of IL-4 (1.69 ± 0.04 ×10 6 ml-1, p<0.001 when compared with the other three groups). The expression levels of MHC-II and CD83 on day 7 after incubation were significantly lower than those on day 3 (MHC-II: p<0.001; CD83: p<0.001). The efficiency of cell fusion under induction of PEG-3000 was significantly higher than that of PEG-4000 (15.4 ± 0.56% vs. 11.1 ± 0.45%, p<0.001).
Conclusions: The largest quantity for mature DCs was stimulated with 1000 U/ml of GM-CSF and 500 U/ml of IL-4 and the highest fusion efficiency was under induction of PEG-3000.
کلیدواژه‌ها
Cell Fusion؛ Dendritic cell؛ Melanoma؛ Polyethylene Glycol
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