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Maturation State and Function of Monocyte Derived Dendritic Cells in Liver Transplant Recipients | ||
| Iranian Journal of Immunology | ||
| مقاله 2، دوره 11، شماره 3، آذر 2014، صفحه 153-165 اصل مقاله (798.36 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2014.16776 | ||
| نویسندگان | ||
| Afsoon Shariat1؛ Mohammad Hossein Karimi2؛ Talat Mokhtariazad3؛ Seyed Mohammad Moazzeni4؛ Bita Geramizadeh2؛ Seyed Ali MalekHosseini5؛ Ramin Yaghobi* 2 | ||
| 1Department of Biology, College of Basic Sciences, Tehran Science and Research Branch, Islamic Azad University, Tehran | ||
| 2Shiraz Transplant Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz | ||
| 3Department of Virology, School of Public Health, Tehran University of Medical Sciences, | ||
| 4Department of Immunology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran | ||
| 5Shiraz Transplant Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Background: Dendritic cells (DCs) are potent antigen presenting cells for triggering of the immune reaction post transplantation. These cells are centrally involved in the initiation of T cell-dependent immune responses. Objective: To compare the level of DC maturation and function in liver transplant recipients with healthy controls. Methods: In this study, twelve peripheral blood samples were selected from six liver transplant patients and six healthy controls. After the generation of DCs from monocytes, expression levels and mean fluorescent intensity (MFI) of several DC maturation markers were evaluated using flowcytometry. Secretion of IL-6, IL-12 and IL-23 proinflammatory cytokines was determined using ELISA. Gene expressions of TLR-2, TLR-4 and IL-23 were analyzed using real-time PCR. Results: DC expression markers including CD83 (p=0.007) and CD86 (p=0.02), as well as secretion of IL-6 (p=0.02) and IL-12 (p=0.007) by DCs were significantly increased in liver transplant patients compared with healthy controls. The MFI of CD86 (p=0.009) and HLA-DR (p= 0.005) expression on DCs was also higher in patients. The expression of TLR-2 transcripts in DCs of patients was higher than that of the controls (p=0.03). Conclusion: Based on these findings, increased frequency of DCs expressing CD83 and CD86, higher expression of CD86, HLA-DR, and TLR-2 as well as elevated secretion of proinflammatory cytokines in DCs of liver transplant recipient's point to the more mature phenotype and active function of DCs in patients compared with controls. | ||
| کلیدواژه‌ها | ||
| Dendritic Cells؛ Liver Transplantation؛ TLR | ||
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آمار تعداد مشاهده مقاله: 1,188 تعداد دریافت فایل اصل مقاله: 954 |
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