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H2-EB1 Molecule Alleviates Allergic Rhinitis Symptoms of H2-Eb1 Knockout Mice | ||
| Iranian Journal of Immunology | ||
| مقاله 4، دوره 12، شماره 4، اسفند 2015، صفحه 263-273 اصل مقاله (1.18 M) | ||
| نوع مقاله: Original Article | ||
| نویسندگان | ||
| Linge Li؛ Bin Hu؛ Juan Feng؛ Yu Zhang؛ Xi Shou؛ Yu Tian؛ Chunrong Jiang؛ Hua Zhang* | ||
| Department of Otorhinolaryngology, the First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang, China | ||
| چکیده | ||
| Background: H2-EB1 molecule which is the homolog of Human HLA-DRB1 is proposed to be associated with allergic rhinitis (AR). Construction of H2-Eb1 knockout animal models provides a tool to elucidate the role of H2-EB1 and AR pathogenesis. Objective: To establish the H2-Eb1 knockout model and investigate the H2-EB1 functions in H2-Eb1 knockout mice as a model of AR. Methods: The Cre/LoxP system and ES gene knockout technology were applied to create heterozygous H2-Eb1 (+/-) knockout mice and their offspring of knockout homozygous(-/-), heterozygous (+/-) and wild type (+/+) H2-Eb1 mice. After identification, offspring of heterozygous (+/-) and homozygous (-/-) H2-Eb1 knockout mice were randomly selected to establish AR models to demonstrate the role of H2-Eb1 in AR pathogenesis. Results: The H2-Eb1 knockout mice model was successfully established. The reproduction and feeding of the homozygous ( -/-) H2-Eb1 knockout mice were successful. Compared with the control group, the serum OVA-IgE and IL-4 levels significantly increased, while IFN-γ levels significantly dropped (p<0.05) in the experimental groups. For the two experimental groups, the homozygous ( -/-) mice group had lower serum OVA-IgE and IL-4 levels, and higher IFN-γ levels than their heterozygous (+/-) counterparts (p<0.05), concomitant with slighter allergic symptoms (gentle behavior and less eosinophils in nasal mucosa). Conclusion: Our study demonstrated that knockout of H2-Eb1 gene could alleviate mouse AR Symptoms, indicating H2-Eb1 may play an important role in regulating Th1/Th2 balance during the pathogenesis of AR. | ||
| کلیدواژهها | ||
| H2-Eb1؛ Allergic Rhinitis؛ Knockout؛ Th1؛ Th2 | ||
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