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Influence of 1 Alpha, 25-Dihydroxyvitamin D3 on T Helper 17 Cells and Related Cytokines in Systemic Lupus Erythematosus | ||
| Iranian Journal of Immunology | ||
| مقاله 1، دوره 12، شماره 2، شهریور 2015، صفحه 82-93 اصل مقاله (509.12 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2015.16738 | ||
| نویسندگان | ||
| Hadi Reihani1؛ Maryam Rastin1؛ Mahmoud Mahmoudi1؛ Mohsen Ghoryani1؛ Nafiseh Abdollahi2؛ Nafiseh Sadat Tabasi1؛ Shahrzad Zamani Taghizadeh Rabe1؛ Maryam Sahebari* 3 | ||
| 1Immunology Research Center, BuAli Research Institute | ||
| 2Bone, Joint and Connective Tissue Research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 3Rheumatic Diseases Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad | ||
| چکیده | ||
| Background: Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease. Emerging data suggests that T helper 17 (Th17) cells play a pathogenic role in SLE and the increased number of these cells correlates with disease activity. In recent years, 1α, 25-dihydroxyvitamin D3 (1,25VitD3) has been considered as an immunomodulatory factor. Objective: To investigate the effect of 1,25VitD3 on Th17 cells and on the expression of related cytokines in SLE patients. Method: Thirty SLE patients (newly diagnosed or in remission) were sampled for 10 ml whole blood to isolate peripheral blood mononuclear cells (PBMCs) using Ficoll-Hypaque density gradient centrifugation. Isolated cells were cultured in the presence and absence of 50 nM 1,25VitD3. After incubation, cells were harvested and stimulated for 4-5 hours with phorbol myristate acetate (PMA) and ionomycin in the presence of brefeldin A. IL-17 secreting cells were analyzed by flowcytometry. RNA was extracted from cultured cells, cDNA was synthesized, and the expression levels of IL-6, IL-17, IL-23 and TGF- β genes were assessed by real-time PCR. Results: The percentage of Th17 cells (CD3+ CD8- IL-17+ T cells) decreased significantly in 1,25VitD3-treated cells (3.67 ± 2.43%) compared to untreated cells (4.65 ± 2.75%) ( p=0.003). The expression of TGF- β up regulated (1.38-fold) and the expression of IL-6 (50%), IL-17 (27%) and IL-23 (64%) down regulated after 1,25VitD3 treatment. Conclusion: This study showed that 1,25VitD3 modulates Th17 related pathways in SLE patients and revealed the immunomodulatory effect of 1,25VitD3 on the Th17 mediated autoimmunity. | ||
| کلیدواژه‌ها | ||
| IL-17؛ IL-23؛ systemic lupus Erythematosus؛ Th17؛ TGF-β؛ Vitamin D | ||
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